Sequences from confirmation amplicon


Confirmation sequences for line 800G05 and hit At3g58500 are shown.
Line ID 800G05
Locus At3g58500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21638728
Primers used for PCR NH76 - 8474, show primer details
   
Sequence Name 64-K102594-0022-800-G05C-8409
GenBank Accession LN511172 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K102594-0022-800-G05C-8409
TTTACATTGAATAATCCTGTGCTTCACTCGAAAAACTAAATTACAGATCCAATTTTAAAC
GAAGAAAACGAAAAGGTGCGAGGAAAGCTATATTCTAACACAATCACAGGCACCTGTTGC
TCGGAGAGAGGCTTGCACTGCATGAGCTGCGAGATCTGCTCATCTAGATCGAGGGTTGCA
TCCGTTGGAAGCGAATTCGCGCCCATGTTTTTGGTAGTGCGTCGTCGTCTTCTTTCCTTC
AAGTCGGATCCGGAAAACAGATCAGAAACGAGGCAAAGGTTTCCAGATTAGGGGCAGCCC
TAACCCTAAATTGGATGGATTCTGCGTATTCTTCGCTTTTTTCTTTCTCTCTCTCTCTCG
CCTTCGTGTGCAGAGTGAGAGAGGAAATCAAAACAAAGGGGCGCGTGAGGTAAAGGGGCG
AGTGGGTTTGTAGCTTTCGAGCTGTGTCTTGATTTGACCATTATATCCT
   
Sequence Name 64-K102595-0022-800-G05C-NH76
GenBank Accession LN511173 [GenBank]
Sequencing primer gene specific primer, NH76: 5'-TCTCAACGAGGAGGATATAATGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K102595-0022-800-G05C-NH76
CTCAACCCCTCGCCCCTTTACCTCACGCGCCCCTTTGTTTTGATTTCCTCTCTCACTCTG
CACACGAAGGCGAGAGAGAGAGAGAAAGAAAAAAGCGAAGAATACGCAGAATCCATCCAA
TTTAGGGTTAGGGCTGCCCCTAATCTGGAAACCTTTGCCTCGTTTCTGATCTGTTTTCCG
GATCCGACTTGAAGGAAAGAAGACGACGACGCACTACCAAAAACATGGGCGCGAATTCGC
TTCCAACGGATGCAACCCTCGATCTAGATGAGCAGATCTCGCAGCTCATGCAGTGCAAGC
CTCTCTCCGAGCAACAGGTGCCTGTGATTGTGTTAGAATATAGCTTTCCTCGCACCTTTT
CGTTTTCTTCGTTTAAAATTGGATCTGTAATTTAGTTTTTCGAGTGAAGCACAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTACATT

Last Updated on 06.12.2018 16:11