Sequences from confirmation amplicon


Confirmation sequences for line 801D05 and hit At3g58350 are shown.
Line ID 801D05
Locus At3g58350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21592586
Primers used for PCR KA02 - 8474, show primer details
   
Sequence Name 59-K105307-0022-801-D05C-KA02
GenBank Accession LN509998 [GenBank]
Sequencing primer gene specific primer, KA02: 5'-TGTTGAAGTTTCCTCTGTCACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K105307-0022-801-D05C-KA02
GACTTTGATTCGACACAATCTTAAGTTCCCCGTTTAACAGAAATCCGCTATCTTTGGCAT
GAATTTCATTTAGGGGACACATGGATGAAAGACCCCAGTTGGTAGTCTTCTCATCAAACC
ACTGTTCTAGTTCTGTAAAATCAATCACACACAAAAAAAAATAATAATAAAAAAAATTAG
AGAGGAACTCTTGCAAAGCTAGCAGTGGTAATAAGTTCTTACCATTAAGTTTTGACTGAG
AGAGTTTATCTGATAATTGATTTACTAGAGTTAGACAAAATTTAGTGTGTCTTCTCCATC
CAGAAGGCAGAGAAGTAGGATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACGGTTATA
   
Sequence Name 43-K105308-0022-801-D05C-8409
GenBank Accession LN504834 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K105308-0022-801-D05C-8409
GCCATTTTCCTACTTCTCTGCCTTCTGGATGGAGAAGACACACTAAATTTCGTCTAACTC
TAGTAAATCAATTATCAGATAAACTCTCTCAGTCAAAACTTAATGGTAAGAACTTATTAC
CACTGCTAGCTTTGCAAGAGTTCCTCTCTAATTTTTTTTATTATTATTTTTTTTTGTGTG
TGATTGATTTTACAGAACTAGAACAGTGGTTTGATGAGAAGACTACCAACTGGGGTCTTT
CATCCATGTGTCCCCTAAATGAAATTCATGCCAAAGATAGCGGATTTCTGTTAAACGGGG
AACTTAAGATTGTTGTCGAGATCAAAGTTCTTGAAACTATTGGCAAATTAGATGTGACAG
AGGAAACTTCAAC

Last Updated on 06.12.2018 16:11