Sequences from confirmation amplicon


Confirmation sequences for line 804B11 and hit At3g04330 are shown.
Line ID 804B11
Locus At3g04330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1146152
Primers used for PCR KM55 - 8474, show primer details
   
Sequence Name 02-K101217-0022-804-B11C-8409
GenBank Accession LN486957 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101217-0022-804-B11C-8409
TGAGTAGTCCTCAACGGATCTGAATCTACGTCAGACAATGCCAGTACGTTCGCCATCGAG
CAAACTAACGAATACTACAAGTTCACTTTCGGTGACGGCCCTGACTATCTAACGACTATC
AGTTTATCCAACGATTATCCAATATATAGGTTGTTATCCAAGAAGTTCTCTGGAGAAATG
GAGATTTATTTCTACAAGAACCTTACTATGGGTTAATTAAGCCATGATGTTTTTATTTCC
AAAATCAAAATAAAACTTGTTGTAAGAACATGAATACGCAATAAAAATGATATAATAACA
TAAAAATAAAACTAAACTCGGAGATCATAAGTTGGTTCATTTACTGTGTATTCATGTTAG
TATTACGCTAATTTAAGATATTTTCTTGTGATATGGGTTACAACTACGTGATGAAGGAAC
CAACTTGATATGACAAAAATCATTTAGAAAAAAACAATGATATAATAACACAAGAAAGAA
CAAATACTCTGAGATATAAGTTGTGCCGTGAGTTACATTGTCAGCAGAGGAAGCAGATTG
ACTTGTTCCCGTCGTGTCACGGTTTTTCTAGACCTTTG
   
Sequence Name 02-K101223-0022-804-B11C-KM55
GenBank Accession LN486958 [GenBank]
Sequencing primer gene specific primer, KM55: 5'-TGGTTCCTCAAAGGTCTAGAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101223-0022-804-B11C-KM55
TCTGCTTCCTCTGCTGACATGTAACTCACGGCACAACTTATATCTCAGAGTATTTGTTCT
TTCTTGTGTTATTATATCATTGTTTTTTTCTAAATGATTTTTGTCATATCAAGTTGGTTC
CTTCATCACGTAGTTGTAACCCATATCACAAGAAAATATCTTAAATTAGCGTAATACTAA
CATGAATACACAGTAAATGAACCAACTTATGATCTCCGAGTTTAGTTTTATTTTTATGTT
ATTATATCATTTTTATTGCGTATTCATGTTCTTACAACAAGTTTTATTTTGATTTTGGAA
ATAAAAACATCATGGCTTAATTAACCCATAGTAAGGTTCTTGTAGAAATAAATCTCCATT
TCTCCAGAGAACTTCTTGGATAACAACCTATATNTTGGATAATCGTTGGATAAACTGATA
GTCGTTAGATAGTCAGGGCCGTCACCGAAAGTGAACTTGTAGTATTCGTTAGTTTGCTCG
ATGGCGAACGTACTGGCATTGTCTGACGTAGATTCAGATCCGTTGAGGACTACTTCACCT
TCTGAAGAANAAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGA

Last Updated on 06.12.2018 16:11