Sequences from confirmation amplicon


Confirmation sequences for line 804C11 and hit At4g10050 are shown.
Line ID 804C11
Locus At4g10050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6285057
Primers used for PCR AH29 - 8474, show primer details
   
Sequence Name 46-K105566-0022-804-C11C-AH29
GenBank Accession LN505866 [GenBank]
Sequencing primer gene specific primer, AH29: 5'-GATTGAATACAGCGTTAGAGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K105566-0022-804-C11C-AH29
CCCCACAACTCTCAATATGATGATTCAAGCATTGGTGCGTATTGTTACTGATTTTTCCTG
TTTTGAGGGTTCACATTTTTGCTTCATAATGAGTGGAAATATGAACCTTTGTCCAGCTAC
GTTTACCGAACACGTCTAGAGGAAACAGAGCAATATTGGAAAGGCTGGTATGACCATCAT
TACCTCTTGTATTCCTGAGACGGTTGAATATTAAGAGTTGTTAGTTGCAACAGTTGCCGT
TGTGAATCTGAGTGCAAACATTTACCTCTCTTGAATAGTTTTTGATTCGAACAATGTTAG
TTGCCCTGCAGGTACGATGGTCTCTCAGAGAAGTTTTTGTCATCCCCAGTTCCGAAACTC
TTGCTATTAGCTGGAACTGATAGGTTGGACAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 78-K105567w-0022-804-C11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K105567w-0022-804-C11C-8409
CATTTACAATTGAATATATCCTGTCCACCTATCAGTTCCAGCTAATAGCAAGAGTTTCGG
AACTGGGGATGACAAAAACTTCTCTGAGAGACCATCGTACCTGCAGGGCAACTAACATTG
TTCGAATCAAAAACTATTCAAGAGAGGTAAATGTTTGCACTCAGATTCACAACGGCAACT
GTTGCAACTAACAACTCTTAATATTCAACCGTCTCAGGAATACAAGAGGTAATGATGGTC
ATACCAGCCTTTCCAATATTGCTCTGTTTCCTCTAGACGTGTTCGGTAAACGTAGCTGGA
CAAAGGTTCATATTTCCACTCATTATGAAGCAAAAATGTGAACCCTCAAAACAGGAAAAA
TCAGTAACAATACGCACCAATGCTTTGAATCATCATATTTGAGAGTTGTGGGGATAGAGA
CACGGGCTGAATCAATGTTTCTTAATGACCCTCCTCTAACGCTGAAATTCAAT

Last Updated on 06.12.2018 16:11