Sequences from confirmation amplicon


Confirmation sequences for line 804E11 and hit At5g58400 are shown.
Line ID 804E11
Locus At5g58400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23605366
Primers used for PCR 14vo - 8474, show primer details
   
Sequence Name 27-K118658-0022-804-E11C-14vo
GenBank Accession LN498609 [GenBank]
Sequencing primer gene specific primer, 14vo: 5'-CTTATTAACCTTTTCCGAGCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118658-0022-804-E11C-14vo
GCGCTCTCCGGGGCTCACACCATTGGGCAAGCCAGGTGCGTCACCTTCAGGAGCCGGATA
TATAACTCAACAAACATCGACCTTTCATTTGCCTTATCCAGACGGAGGAGCTGCCCTGCG
GCAACTGGCTCCGGCGATAACAACGCCGCCATTCTCGATCTCCGCACACCTGAAAAGTTC
GACGGCAGCTACTTCATGCAGCTTGTCAACCACAGGGGACTTCTCACATCAGATCAGGTC
TTGTTCAACGGAGGTTCCACCGACTCGATCGTCGTATCCTACAGCCGGAGTGTCCAAGCA
TTCTACAGAGATTTTGTAGCGGCGATGATAAAAATGGGCGACATCAGCCCACTCACCGGG
TCCAATGGACAGATCCGTCGCAGCTGTCGCAGGTCCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAAGTCCGCACGGTTTATTA
TAT
   
Sequence Name 12-K118657-0022-804-E11C-8409
GenBank Accession LN491801 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118657-0022-804-E11C-8409
TTTCATTGAATATATCCTGGACCTGCGACAGCTGCGACGGATCTGTCCATTGGACCCGGT
GAGTGGGCTGATGTCGCCCATTTTTATCATCGCCGCTACAAAATCTCTGTAGAATGCTTG
GACACTCCGGCTGTAGGATACGACGATCGAGTCGGTGGAACCTCCGTTGAACAAGACCTG
ATCTGATGTGAGAAGTCCCCTGTGGTTGACAAGCTGCATGAAGTAGCTGCCGTCGAACTT
TTCAGGTGTGCGGAGATCGAGAATGGCGGCGTTGTTATCGCCGGAGCCAGTTGCCGCAGG
GCAGCTCCTCCGTCTGGATAAGGCAAATGAAAGGTCGATGTTTGTTGAGTTATATATCCG
GCTCCTGAAGGTGACGCACCTGGCTTGCCCAATGGTGTGAGCCCCGGAGAGCGCCACCAT
GTCGCGTGGGGATAAACCGTTTGCTCGGAAAGGTTTAATAA

Last Updated on 06.12.2018 16:11