Sequences from confirmation amplicon


Confirmation sequences for line 805C08 and hit At3g23120 are shown.
Line ID 805C08
Locus At3g23120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8227806
Primers used for PCR DA22 - 8474, show primer details
   
Sequence Name 56-K108477-0022-805-C08C-DA22
GenBank Accession LN509126 [GenBank]
Sequencing primer gene specific primer, DA22: 5'-TCTTTGATGAACTGCCAAGATATG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K108477-0022-805-C08C-DA22
AGACAGATCAAGACCGTTTCATTTTGGTTGGGCTCTCGGAAATCATTAATGGTTCTTGTG
CTCCGATCAAATGCATTCTATGGTCCAGTCTATAACTCCACTACCTATTTGGGGTTTCCA
CGCTTGAGTATCATTGATATATCTAATAACGATTTCGTTGGGTCATTACCACAAGACTAT
TTTGCCAATTGGACTGAAATGGCAACGGTGTGGGACATCAATCGACTGAACTACGCAAGA
AACACTTCTTCAAGAACAATACAATATGGGGGACTTCAAACTATCCAAAGGTCGAACTAC
GTGGGGGATAATTTCAATATGCATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 40-K108476-0022-805-C08C-8409
GenBank Accession LN503732 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K108476-0022-805-C08C-8409
ATTTACATTGAATTATCCTGCATGCATATTGAAATTATCCCCCACGTACTTTGACCTTTG
GATAGTTTGAAGTCCCCCATATTGTATTGTTCTTGAAGAAGTGTTTCTTGCGTAGTTCAG
TCGATTGATGTCCCACACCGTTGCCATTTCAGTCCAATTGGCAAAATAGTCTTGTGGTAA
TGACCCAACGAAATCGTTATTAGATATATCAATGATACTCAAGCGTGGAAACCCCAAATA
GGTAGTGGAGTTATAGACTGGACCATAGAATGCATTTGATCGGAGCACAAGAACCATTAA
TGATTTCCGAGAGCCCAACCAAAATGGAAACGTGTCTTTGATCTTGTTTCCTCTCACATT
CAAAAATTCCATATCTTGGCAGTTCATCAAAGA

Last Updated on 06.12.2018 16:11