Sequences from confirmation amplicon


Confirmation sequences for line 806A11 and hit At5g60070 are shown.
Line ID 806A11
Locus At5g60070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24192190
Primers used for PCR DA23 - 8474, show primer details
   
Sequence Name 14-K106018-0022-806-A11C-DA23
GenBank Accession LN492512 [GenBank]
Sequencing primer gene specific primer, DA23: 5'-ATGTCCCTTCATTAACTCAACCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K106018-0022-806-A11C-DA23
TGTGAGTGGCGTCTGACCCTTTTTATCAGTCCTCGTTGCCGTGTCCGGTTCCACCGCCAC
AATCGCTTTTACCACTTCCGCGTGACCATTCCTAGCCGCCGAGTGCAATGCTGTTTTCCC
GTTGCTTTTTGCGATCGCGGCTAGGCTACTCCCTGCTGCTTCTAGTAGATACTCTACGAC
CTCTACGTGTCCCTGAGCTGCCGCCGTGTGTAGAGCCGTCGTGTTTGATAAGTCCACCGT
CATTGATAGCTCCGGATGTTCTTCCATTAGTACTCTCAAAACATCTATAAAAATAGATTT
CAAATTTCATCAATGTATCACACATGTAAATAAATATAGAATATTATCATCAGTTTTTCT
TTAATTCTTTCCATTCAATTCTTTAAATCAACCAAAGATTAACACTTTAACCATATTTTC
GTGTAATTTTTTCTTCCCAATTCTTAAGAACAAACAAAGATAGGTAGTTTTAAGTTCAGT
TCTTAATATCAAGCAAACATTGAAATTTTCATCATTTTGGATAGAAGAAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 14-K106019-0022-806-A11C-8409
GenBank Accession LN492513 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K106019-0022-806-A11C-8409
ATTTTCTTCTTCCAAATGATGAAAATTTCAATGTTTGCTTGATACTATAAACTGAACTTA
AAACTACCTATCTTTGTTTGTTCTTAAGAATTGGGAAGAAAAAATTACACGAAAATATGG
TTAAAGTGTTAATCTTTGGTTGATTTAAAGAATTGAATGGAAAGAATTAAAGAAAAACTG
ATGATAATATTCTATATTTATTTACATGTGTGATACATTGATGAAATTTGAAATCTATTT
TTATAGATGTTTTGAGAGTACTAATGGAAGAACATCCGGAGCTATCAATGACGGTGGACT
TATCAAACACGACGGCTCTACACACGGCGGCAGCTCAGGGACACGTAGAGGTCGTAGAGT
ATCTACTAGAAGCAGCAGGGAGTAGCCTAGCCGCGATCGCAAAAAGCAACGGGAAAACAG
CATTGCACTCGGCGGCTAGGAATGGTCACGCGGAAGTGGTAAAAGCGATTGTGGCGGTGG
AACCGGACACGGCAACGAGGACTGATAAAAAGGGTCAGACGCCACTTCACATGGCGGTGA
AAGGACAAAGCATTGATGTGGTGGTTGAGTTAATGAAGGGACA

Last Updated on 06.12.2018 16:11