Sequences from confirmation amplicon


Confirmation sequences for line 806D01 and hit At5g64220 are shown.
Line ID 806D01
Locus At5g64220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25689621
Primers used for PCR 05nj - 8474, show primer details
   
Sequence Name 82-K114626-0022-806-D01C-05nj
GenBank Accession LN514223 [GenBank]
Sequencing primer gene specific primer, 05nj: 5'-TGTATTGGCACCATAAATGTCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K114626-0022-806-D01C-05nj
AATCGAATTTCTCGTACTTCCTGCAAGAAAATCTGTCAGAACATGTTATATAGAACGGGA
CTCGACCGACTTCATGTGGCGGAGCATGACAACAAAGAACACCATCTACTAAAATATCAG
CCGGAACCTCCACTTCTCCAAACATGCATGACCAGCTATAGCTAGTTACTTCCTGAGGAC
TCAACAGAAATGTCCCGATAACCATTACCTGTTCGAAAATTAAAATGGTAGATGAGAAAG
GAGTTATCTCCTAACAAACTTCCAAAGCCAAAGAGCTCAAAAATAGGAAGAAACAGAGCA
GAACAGCAGAAAGAAAGTACTGTTCCTAAACGTATACNGGCTTACTACTTGCAGAAAGAT
TGATTATCAAAGGTGTTATACGAAAAAAAAATCTTCATTTAAGTTTGGCAAATTGGATTA
GCCAAAGACCATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 82-K114625-0022-806-D01C-8409
GenBank Accession LN514222 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K114625-0022-806-D01C-8409
ATTTGCCAACTTAAATGAAGATTTTTTTTTCGTATAACACCTTTGATAATCAATCTTTCT
GCAAGTAGTAAGCCTGTATACGTTTAGGAACAGTACTTTCTTTCTGCTGTTCTGCTCTGT
TTCTTCCTATTTTTGAGCTCTTTGGCTTTGGAAGTTTGTTAGGAGATAACTCCTTTCTCA
TCTACCATTTTAATTTTCGAACAGGTAATGGTTATCGGGACATTTCTGTTGAGTCCTCAG
GAAGTAACTAGCTATAGCTGGTCATGCATGTTTGGAGAAGTGGAGGTTCCGGCTGATATT
TTAGTAGATGGTGTTCTTTGTTGTCATGCTCCGCCACATGAAGTCGGTCGAGTCCCGTTC
TATATAACATGTTCTGACAGATTTTCTTGCAGTGAAGTACGAGAATTCGATTTCCTTCCT
GGGTCTACCAGAAAGTTAAATGCTACTGACATTTAT

Last Updated on 06.12.2018 16:11