Sequences from confirmation amplicon


Confirmation sequences for line 806D06 and hit At1g12910 are shown.
Line ID 806D06
Locus At1g12910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4395314
Primers used for PCR HP61 - 8474, show primer details
   
Sequence Name 69-K033720-022-806-D06C-8409
GenBank Accession LN512135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K033720-022-806-D06C-8409
ATTTACATTGAAGAGCTATGAGGAGCCCAGCAATGGCATTGACACTAGCTTGATGTCGTT
GAAGCTCAACCACAGGAAGAGCCGCGAAAACGAATGTCAAGCACCACAACTTTAGCACTG
TCCATGATAATAGTAGCCATGTACCTAGGATCCTGTTTGTTCCAACCAAGACGCACTAAA
GGAGTATCAGGCTCGGAGCTCTCATAGATAATCGTCGAATGTTCCTTATCACGAAGATCA
AACACCCTAACGGAGCCATCAGCTGAAACAGATGCAAAAACACCAACACCACCCCAAGCA
ATATCAAAAACTTCCTTATCATGAGCAATAAGCTGAGTATCAACAGCTTCACGCTCAATG
TCCCAGATAGTACAAGTCGTATCAGTACTAGATGTTCCAATTCGACGTGGCTCAGCTTCA
TTCCAATCAAAAGAAGTAAGAGGACCACAAAACTNCACTGTTCT
   
Sequence Name 31-K033720-022-806-D06C-HP61
GenBank Accession LN500061 [GenBank]
Sequencing primer gene specific primer, HP61: 5'-AAGAACAGTGAGTTTTGTGGTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K033720-022-806-D06C-HP61
GTCGAATTGGAACATCTAGTACTGATACGACTTGTACTATCTGGGACATTGAGCGTGAAG
CTGTTGATACTCAGCTTATTCCTCATGATAAGGAAGTTTTTGATATTGCTTGGGGTGGTG
TTGGTGTTTTTGCATCTGTTTCAGCTGATGGCTCCGTTAGGGTGTTTGATCTTCGTGATA
AGGAACATTCGACGATTATCTATGAGAGCTCCGAGCCTGATACTCCTTTAGTGCGTCTTG
GTTGGAACAAACAGGATCCTAGGTACATGGCTACTATTATCATGGACAGTGCTAAAGTTG
TGGTGCTTGACATTCGTTTTCCGGCTCTTCCTGTGGTTGAGCTTCAACGACATCAAGCTA
GTGTCAATGCCATTGCTTGGGCTCCTCATAGCTCTTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11