Sequences from confirmation amplicon


Confirmation sequences for line 806H09 and hit At5g59450 are shown.
Line ID 806H09
Locus At5g59450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23974536
Primers used for PCR NB18 - 8474, show primer details
   
Sequence Name 86-K101387-0022-806-H09C-8409
GenBank Accession LN514632 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K101387-0022-806-H09C-8409
ATTTACAATTGAATATATCCTGTAAAACATACATAATTTAAGAGGTTAGTTAATTTGTGT
CGGATCTATCTGCAACTACTTCTCTAGGGTTTTGGAGTTTGATTCTTGTGTTGTTATCTT
TTTTACTTATCTAATATCTTCCCACTTGCATTGCTTGGTCATGACTAAAAGTCAGGTTCC
TTTACTCAGCTTTGAGAATTGAATTTAAAATATAAAAAAATCATCCAATCTCACATATTT
TCTAATAAACATTTTCACCAATGGAATCATTTTGATAAAATATCAGGTGGATGTATATTG
GCTTGCATCTGATTCCTTATGTGGTTTTGG
   
Sequence Name 86-K101393-0022-806-H09C-NB18
GenBank Accession LN514633 [GenBank]
Sequencing primer gene specific primer, NB18: 5'-ATCCAAAACCACATAAGGAATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K101393-0022-806-H09C-NB18
CCCTGAATTTATCAAATGATTCCATTGGTGAAAATGTTTATTAGAAAATATGTGAGATTG
GATGATTTTTTTATATTTTAAATTCAATTCTCAAAGCTGAGTAAAGGAACCTGACTTTTA
GTCATGACCAAGCAATGCAAGTGGGAAGATATTAGATAAGTAAAAAAGATAACAACACAA
GAATCAAACTCCAAAACCCTAGAGAAGTAGTTGCAGATAGATCCGACACAAATTAACTAA
CCTCTTAAATTATGTATGTTTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCNAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11