Sequences from confirmation amplicon


Confirmation sequences for line 807G10 and hit At5g48655 are shown.
Line ID 807G10
Locus At5g48655 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19731772
Primers used for PCR JF23 - 8474, show primer details
   
Sequence Name 60-K102504-0022-807-G10C-8409
GenBank Accession LN510223 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K102504-0022-807-G10C-8409
TTTACATTGAATAATCCTGAAAGTAATGAGTATATGTAAATGGCTTCACCTCCACCAGAA
GAGCCAAAGTTTACATGTCCAATCTGCATGTGTCCCTTTACGGAGGAGATGTCAACCAAG
TGCGGTCACATCTTCTGCAAGGGATGTATAAAGATGGCAATATCTCGCCAGGGCAAATGC
CCTACTTGTAGGAAAAAGGTTACTGCAAAAGAGCTGATTCGAGTTTTCCTTCCAACCACT
AGATGAGTGGTCCGGCAACATCACCAGCCACCCTGTAGGTCAAACAATGATTATATTATC
ATCCACGTTTGTAGAGATTTACTAGATTTGAATTGTATACTTAATTAACTAAACTGGAAT
TTGGTTTCCATT
   
Sequence Name 60-K102505-0022-807-G10C-JF23
GenBank Accession LN510224 [GenBank]
Sequencing primer gene specific primer, JF23: 5'-GAATGGAAACCAAATTCCAGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K102505-0022-807-G10C-JF23
ATCTCTACAACGCTGGATGATAATATAATCATTGGTTTGACCTACAGGGNTGGCTGGTGA
TGTTGCCGGACCACTCATCTAGTGGTTGGAAGGAAAACTCGAATCAGCTCTTTTGCAGTA
ACCCTTTTTCCTACAAGTAGGGCATTTGCCCTGGCGAGATATTGCCATCTTTATACATCC
CTTGCAGAAGATGTGACCGCACTTGGTTGACATCTCCTCCGTAAAGGGACACATGCAGAT
TGGACATGTAAACTTTGGCTCTTCTGGTGGAGGTGAAGCCATTTACATATACTCATTACT
TTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11