Sequences from confirmation amplicon


Confirmation sequences for line 807H12 and hit At2g43200 are shown.
Line ID 807H12
Locus At2g43200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17959232
Primers used for PCR GE46 - 8474, show primer details
   
Sequence Name 86-K100942-0022-807-H12C-8409
GenBank Accession LN514626 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K100942-0022-807-H12C-8409
TATAAACATTACCATAGGAAGTCCAATTGACAAGGCATCTAGAACAATGGACCATGTCAA
AAGACCTCGACGGGTAAGGCAGCTTATAAGTGCTTAAAACACCGAGCATAGCAGGGAGTC
CACGTTCTAATGCGAACTGAACTTGAGCCTCATGTATATCCCTCGGTGCAATGGACATTG
TCAAAATCTTATAGTTCAGCAAAAAGGCTCCAAAACTCGCTACCTGACATTTTGGGTCCA
ACAAAACACATGGAATCCATCATAATCGAAAGTAGTAATACTGCTGTAAAATATCAAGAT
GAATCAAGTTCTGGTGTCTATTCATGTAACTGAACTCACTCCACATCCAATATCGAGAAC
AGTCCTGATACTCCCAGAAG
   
Sequence Name 86-K100950-0022-807-H12C-GE46
GenBank Accession LN514627 [GenBank]
Sequencing primer gene specific primer, GE46: 5'-CTTCTGGGAGTATCAGGACTGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K100950-0022-807-H12C-GE46
TGAATAGACACCAGAACTTGATTCATCTTGATATTTTACAGCAGTATTACTACTTTCGAT
TATGATGGATTCCATGTGTTTTGTTGGACCCAAAATGTCAGGTAGCGAGTTTTGGAGCCT
TTTTGCTGAACTATAAGATTTTGACAATGTCCATTGCACCGAGGGATATACATGAGGCTC
AAGTTCAGTTCGCATTAGAACGTGGACTCCCTGCTATGCTCGGTGTTTTAAGCACTTATA
AGCTGCCTTACCCGTCGAGGTCTTTTGACATGGTCCATTGTTCTAGATGCCTTGTCAATT
GGACTTCCTATGGTAATGTTTATATTAGACTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11