Sequences from confirmation amplicon


Confirmation sequences for line 808G05 and hit At3g47500 are shown.
Line ID 808G05
Locus At3g47500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17505222
Primers used for PCR CP47 - 8474, show primer details
   
Sequence Name 66-K031140-022-808-G05C-CP47
GenBank Accession LN511521 [GenBank]
Sequencing primer gene specific primer, CP47: 5'-GTTCATTTGTGCTTCAACAACACT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K031140-022-808-G05C-CP47
GCACAGAGGATCCGCTTGAGCAATCATCTCCATTCTCTACCCGAGCTACAAGCCGTTGAT
CCGCTAACCCGTGAAACCTGTTCCTAGCACCGTTTGAGACCTTTTGATCTTCTTGTAGCT
TCATCACAGGTGTCATGGGAGCAGCAACGTGCTGCTGCTGAGCTTCGAGACCAAAACTCA
AGACCCTTGTGTTTGCCTGTAAGCCCGGGTCAAGCCTCGCAGCCTCAAGAGCCTCGGAAA
TAGTGATGTGACGGTAATGAGAAGATGAGCTTTTGTTCTTACGACGTCCTGCCCCCACAG
GAACATTCCTCATAGTCCCTCCAGCAGTCCAATATCTCTGACAAGCCTTGCAGAAATGAC
TTCAAATGTAGAAAAAGAAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAAATGG
   
Sequence Name 31-K031140-022-808-G05C-8409
GenBank Accession LN500040 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K031140-022-808-G05C-8409
TTACATTGAATAATCCTTTCTTTTTCTACATTTGAAGTCATTTCTGCAAGGCTTGTCAGA
GATATTGGACTGCTGGAGGGACTATGAGGAATGTTCCTGTGGGGGCAGGACGTCGTAAGA
ACAAAAGCTCATCTTCTCATTACCGTCACATCACTATTTCCGAGGCTCTTGAGGCTGCGA
GGCTTGACCCGGGCTTACAGGCAAACACAAGGGTCTTGAGTTTTGGTCTCGAAGCTCAGC
AGCAGCACGTTGCTGCTCCCATGACACCTGTGATGAAGCTACAAGAAGATCAAAAGGTCT
CAAACGGTGCTAGGAACAGGTTTCACGGGTTAGCGGATCAACGGCTTGTAGCTCGGGTAG
AGAATGGAGATGATTGCTCAAGCGGATCCTCTGTGACCACCTCTAACAATCACTCAGTGG
ATGAATCAAGAGCACAAAGCGGCAGTGTTGTTGAAGCACAAATGAA

Last Updated on 06.12.2018 16:11