Sequences from confirmation amplicon


Confirmation sequences for line 808H11 and hit At4g14660 are shown.
Line ID 808H11
Locus At4g14660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8406744
Primers used for PCR 17ak - 8474, show primer details
   
Sequence Name 85-K108479-0022-808-H11C-17ak
GenBank Accession LN514556 [GenBank]
Sequencing primer gene specific primer, 17ak: 5'-TATGTGACATTGCTTTGATTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K108479-0022-808-H11C-17ak
GGAACGTGATGATTCCAGCTGAAAACATGGATGCCAAAGGGCTGATGTTGAAGAGAGCTA
TACTAGTAGAGTTACTAGAGGCGTTTGCTTCCAAGAAAGCAACCAAGGAGCTTGGCTACT
ACGTGGCAGTCACAACTTTGGACAAGATTGGAGAAGGCAAAATCAGGGAGCACACCGGTG
AAGTTTTGTTCCCGGTAATGTTCAGCGGAATGACTTTCAAGATCTTCAAAGGAGAGATAA
TTCACGGTGTGGTGCACAAGGTGTTGAAGCATCACTCATCACCATCGAGAAATCTTTAGA
TGGTCTAAATATCCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 73-K108478-0022-808-H11C-8409
GenBank Accession LN512915 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K108478-0022-808-H11C-8409
GCCATTTCATGGATATTTAGACCATCTAAAGATTTCTCGATGGTGATGAGTGATGTTTCA
ACACCTTGTGCACCACACCGTGAATTATCTCTCCTTTGAAGATCTTGAAAGTCATTCCGC
TGAACATTACCGGGAACAAAACTTCACCGGTGTGCTCCCTGATTTTGCCTTCTCCAATCT
TGTCCAAAGTTGTGACTGCCACGTAGTAGCCAAGCTCCTTGGTTGCTTTCTTGGAAGCAA
ACGCCTCTAGTAACTCTACTAGTATAGCTCTCTTCAACATCAGCCCTTTGGCATCCATGT
TTTCAGCTGGAATCATCACGTTCCATGGTAATTGGACTTTGAGAAACATTGTTCAACCTT
CAAAACCAAATCAAAGCAATGTCACAT

Last Updated on 06.12.2018 16:11