Sequences from confirmation amplicon


Confirmation sequences for line 809A07 and hit At1g76800 are shown.
Line ID 809A07
Locus At1g76800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28829658
Primers used for PCR 55pm - 8474, show primer details
   
Sequence Name 62-K115259-0022-809-A07C-55pm
GenBank Accession LN510823 [GenBank]
Sequencing primer gene specific primer, 55pm: 5'-GAGAAAACTTTGTTGCAGATTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K115259-0022-809-A07C-55pm
ACCGACTACAACTAAAAATAATATTTTCAAGGTACAAATTAATGGCCAAAAAAGAAAAAC
AATTAGTGGTGATGATGATGCTCCACATATTGTCTCCACCAAGCCAAAAAGTAAAACACA
AAAATCTCCATAAATAGAACTGAGCCTAGGCGTGTGTTCTCCACACTTACTCGATTCTCT
ATATATATCTCAACAAAACAAAACAAACAAAGAAACAATGGATCAATCAGGTAGTAACAC
GAACATGGACATAGAGAAGGAATCAACTACATTTGATTACTCCAAACGTTCCCAATGGCT
ACGTGCGGCCGTGCTCGGAGCCAACGATGGTCTAGTCTCCACCGCGTCGTTGATGATGGG
AGTTGGAGCTGTGAAGCATGACGTCAAGGCAATGATTCTCTCCGGATTCGCAGGGATGGT
GGCAGGAGCTTGTAGTATGGCCATAGGAGAGTTCGTCTCAGTTTACTCTCAGTACGATAT
AGAGGTGGCTCAGATGGAGAGAGACAGTGTGGAGATAGAGAAAGAGAAGCTTCCTAGTCC
GATGCAAGCCGCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACACA
   
Sequence Name 22-K115258-0022-809-A07C-8409
GenBank Accession LN496240 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115258-0022-809-A07C-8409
AGCTTCTCTTTCTCTATCTCCACACTGTCTCTCTCCATCTGAGCCACCTCTATATCGTAC
TGAGAGTAAACTGAGACGAACTCTCCTATGGCCATACTACAAGCTCCTGCCACCATCCCT
GCGAATCCGGAGAGAATCATTGCCTTGACGTCATGCTTCACAGCTCCAACTCCCATCATC
AACGACGCGGTGGAGACTAGACCATCGTTGGCTCCGAGCACGGCCGCACGTAGCCATTGG
GAACGTTTGGAGTAATCAAATGTAGTTGATTCCTTCTCTATGTCCATGTTCGTGTTACTA
CCTGATTGATCCATTGTTTCTTTGTTTGTTTTGTTTTGTTGAGATATATATAGAGAATCG
AGTAAGTGTGGAGAACACACGCCTAGGCTCAGTTCTATTTATGGAGATTTTTGTGTTTTA
CTTTTTGGCTTGGTGGAGACAATATGTGGAGCATCATCATCACCACTAATTGTTTTTCTT
TTTTGGCCATTAATTTGTTACCTTGAAAATATTATTTTTTAGTTTGTAGTCGGTGCATAA
CGACATAAATTTTTCATATTTATGACGGAAATCTGCA

Last Updated on 06.12.2018 16:11