Sequences from confirmation amplicon


Confirmation sequences for line 809B08 and hit At5g08300 are shown.
Line ID 809B08
Locus At5g08300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2667934
Primers used for PCR JF22 - 8474, show primer details
   
Sequence Name 61-K102504-0022-809-B08C-8409
GenBank Accession LN510483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K102504-0022-809-B08C-8409
CATTTACATTGAATATATATGCTTTTTCATCACCTAAATTCCATTAGATCCTGGAGATGA
GAGATTGCTTTAAAGCAAGTGGCTTTACTAATTTACGTTGTGTGCGTTTTTATTGTGTAA
TAATAGGTGGCAGGAGTGACACCGAAGAAGGGTGGAACAGAACACTTGGGTCTCCCAGTT
TTCAACTCTGTTGCTGAAGCTAAGGCTGACACCAAAGCCAATGCCTCTGTGATTTATGTT
CCTGCTCCTTTTGCTGCTGCTGCTATTATGGAGGGTATTGAGGCTGAGTTAGATCTTATT
GTGTGTATCACCGAAGGA
   
Sequence Name 61-K102505-0022-809-B08C-JF22
GenBank Accession LN510484 [GenBank]
Sequencing primer gene specific primer, JF22: 5'-TTCCTTCGGTGATACACACAATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K102505-0022-809-B08C-JF22
CAGCAGCAGCAAAGGAGCAGGAACATAAATCACAGAGGCATTGGCTTTGGTGTCAGCCTT
AGCTTCAGCAACAGAGTTGAAAACTGGGAGACCCAAGTGTTCTGTTCCACCCTTCTTCGG
TGTCACTCCTGCCACCTATTATTACACAATAAAAACGCACACAACGTAAATTAGTAAAGC
CACTTGCTTTAAAGCAATCTCTCATCTCCAGGATCTAATGGAATTTAGGTGATGAAAAAG
CATATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11