Sequences from confirmation amplicon


Confirmation sequences for line 809B09 and hit At3g05780 are shown.
Line ID 809B09
Locus At3g05780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1716545
Primers used for PCR 71pm - 8474, show primer details
   
Sequence Name 38-K115331-0022-809-B09C-71pm
GenBank Accession LN503095 [GenBank]
Sequencing primer gene specific primer, 71pm: 5'-AGTAAATAGTTTGAGATTCAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115331-0022-809-B09C-71pm
TTACGTAATCTTCTCATCTGCTTTTGGAGATTTCTAACTCCTGCTTCTCTACATAATTTT
CTATCAAGGAAAGAAGAGCTGCATCACTCAAATCAACATGTTCAGGCTTAATGCCGCAAT
CTCTGCATGTCTTTTTCACCAAATAGTCTCTAGCAATATGCATTTTCTCATCAGTAACAT
ACCCAGAGAGATCAATCACCTCCATTCTGTCTAGCAGAGGACCCGGAATCATTTCTATCA
CATTTGCAGTGCATACAAATAAAACCTGCAATAAATTAGTTTAGACCTTACGTCAAACAC
AAAACTGCATATCATGAATATCAATACACAGAGTTTTCCTGAACCGTGATGGATCAAAAA
ATTGCAAAGAGTAGAAAAAACCGCCATGGCAAAAGAGTAAAAAAACCTTTGATAAGTCAA
TAGTAACATTGAGAAAGTGGTCTAAAAATTTTGCATTCTGCTCTGGATCCATAACCTCCA
ACAACGCANTGGCTGGGTCGCCAGTGTGGCACCTACCAAGCTGCCAAGGAACAGCAGACA
AATTATTGCAAAAGCGTAACTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCACAAATGTAGATGTCCGCA
   
Sequence Name 14-K115330-0022-809-B09C-8409
GenBank Accession LN492638 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115330-0022-809-B09C-8409
ATTTCATTGAATATATCCGTTACGCTTTTGCAATAATTTGTCTGCTGTTCCTTGGCAGCT
TGGTAGGTGCCACACTGGCGACCCAGCCAGTGCGTTGTTGGAGGTTATGGATCCAGAGCA
GAATGCAAAATTTTTAGACCACTTTCTCAATGTTACTATTGACTTATCAAAGGTTTTTTT
ACTCTTTTGCCATGGCGGTTTTTTCTACTCTTTGCAATTTTTTGATCCATCACGGTTCAG
GAAAACTCTGTGTATTGATATTCATGATATGCAGTTTTGTGTTTGACGTAAGGTCTAAAC
TAATTTATTGCAGGTTTTATTTGTATGCACTGCAAATGTGATAGAAATGATTCCGGGTCC
TCTGCTAGACAGAATGGAGGTGATTGATCTCTCTGGGTATGTTACTGATGAGAAAATGCA
TATTGCTAGAGACTATTTGGTGAAAAAGACATGCAGAGATTGCGGCATTAAGCCTGAACA
TGTTGATTTGAGTGATGCAGCTCTTCTTTCCTTGATAGAAAATTATTGTAGAGAAGCAGG
AGTTAGAAATCTCCAAAAGCAGATTGAGAAGATTTACCGTAAGGTAAGTTGCTACAAAGT
CGCAATGTCGCATGTTTCTTGAATCTCNAACTATTTAC

Last Updated on 06.12.2018 16:11