Sequences from confirmation amplicon


Confirmation sequences for line 809D12 and hit At1g18910 are shown.
Line ID 809D12
Locus At1g18910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6533228
Primers used for PCR GA44 - 8474, show primer details
   
Sequence Name 72-K101386-0022-809-D12C-8409
GenBank Accession LN512692 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K101386-0022-809-D12C-8409
CTTTACAATTGAATATTCATGTTTATGGTTGTAGGAGTACACATGTTCGCATTACACATG
CCCTATTTGCAGCAAGTCGCTCGGGGATATGCAGGTTAGATACTCCAAAGTCTGGTTCTC
TCATTAAACAATTACTAATAATAGTCGCGTAAAAGATAGCTAATGGGAGCATTTTATCAA
ATGCTCCTAAAAAGAAAAATCTCAACGTATACNGTGATGATCAAACACTGAGGTGATTCA
CTAATCAAAGAACTGTCTTCTCTCACGCAGGTTTACTTTAGAATGTTAGACGCACTGCTT
GCAGAACAAAAGATGCCAGATGAATACTTAAACCAAACTCAGGTTCATCTTTCGTTCTCA
CACACTGATACTTAATGCANATTATCAGTTCCATTGTCTAAATNTAACTTAAGCATATGG
ATAAATGGATGCAGGTNATACTGTGTAATGACTGT
   
Sequence Name 72-K101392-0022-809-D12C-GA44
GenBank Accession LN512693 [GenBank]
Sequencing primer gene specific primer, GA44: 5'-CCTTTCTTCCACAGTCATTACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K101392-0022-809-D12C-GA44
CTTAGTTAAATTTAGACATGGAACTGATAATTTGCATTAAGTATCAGTGTGTGAGAACGA
AAGATGAACCTGAGTTTGGTTTAAGTATTCATCTGGCATCTTTTGTTCTGCAAGCAGTGC
GTCTAACATTCTAAAGTAAACCTGCGTGAGAGAAGACAGTTCTTTGATTAGTGAATCACC
TCAGTGTTTGATCATCACTGTATACGTTGAGATTTTTCTTTTTAGGAGCATTTGATAAAA
TGCTCCCATTAGCTATCTTTTACGCGACTATNATTAGTAATTGTTTAATGAGAGAACCAG
ACTTTGGAGTATCTAACCTGCATATCCCCGAGCGACTTGCTGCAAATAGGGCATGTGTAA
TGCGAACATGTGTACTCCTACNACCATAAACATGAATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCA
Primers used for PCR AF10 - 3144, show primer details
   
Sequence Name 20-K105138-0022-809-D12C-AF10
GenBank Accession LN495207 [GenBank]
Sequencing primer gene specific primer, AF10: 5'-AACTGGAGTAGTTTTTGACGGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K105138-0022-809-D12C-AF10
TACTGCAATCTCTGCCGGCTAGGAAAAGGACTGAGCATAGACTACTTTCATTGCATGAAA
TGTAATGCTTGTATGTCGCGACTCATAGTAGAACATGTATGCAGAGAAAAGTGCTTAGAA
GATAATTGTCCGATTTGTCATGAATACATATTTACCTCAAATTCTCCGGTGAAGGCTCTT
CCATGCGGTCACGTGATGCATTCAACATGCTTTCAGGTGCTTTACTTTCTCGGTTTGTTA
TATGCAATGATGAAACACTTGATACACATTTGCTAAGAATGTTACTTAAGGGATATATTC
AATTGTAAATGGCTTCATGTAAATGGATAAGAATGTTACTTAAGGGATATATTCCCGGAC
AGGATATATTCAATTGTAAATGGCTTTTGCTGTGAGCCATTTAATATATCCCATCATACT
CTTAGCAAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTA
CCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATA
GTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATC
   
Sequence Name 04-K105139-0022-809-D12C-3144
GenBank Accession LN487912 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K105139-0022-809-D12C-3144
TTCGCAGACCCTTCCTCTNATAAGGAAGTTCATTTCATTTGCAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTTGCTAAGAGTATGA
TGGGATATATTAAATGGCTCACAGCAAAAGCCATTTACAATTGAATATATCCTGTCCGGG
AATATATCCCTTAAGTAACATTCTTATCCATTTACATGAAGCCATTTACAATTGAATATA
TCCCTTAAGTAACATTCTTAGCAAATGTGTATCAAGTGTTTCATCATTGCATATAACAAA
CCGAGAAAGTAAAGCACCTGAAAGCATGTTGAATGCATCACGTGACCGCATGGAAGAGCC
TTCACCGGAGAATTTGAGGTAAATATGTATTCATGACAAATCGGACAATTATCTTCTAAG
CACTTTTCTCTGCATACATGTTCTACTATGAGTCGCGACATACAAGCATTACATTTCATG
CAATGAAAGTAGTCTATGCTCAGTCCTTTTCCTAGCCGGCAGAGATTGCAGTATGGACAA
TGATAGATTTCCCTGCAAAGAAAACCTTTAGCCGTCAAAA

Last Updated on 06.12.2018 16:11