Sequences from confirmation amplicon


Confirmation sequences for line 809G04 and hit At4g18690 are shown.
Line ID 809G04
Locus At4g18690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10282950
Primers used for PCR 34ai - 8474, show primer details
   
Sequence Name 10-K106771-0022-809-G04C-8409
GenBank Accession LN490674 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K106771-0022-809-G04C-8409
TTGAATAATCCTGCCGCCGCTGCCGCTTTGCACCCGATTTTACCAACGAGTTCTTCAAGC
TTGTGATCTTCTTTGGATCGATGACTCATGAGTGCTTCTTTGAGATCAACTATGTGTTTA
GCCTGCACACTCATCCACTCATAGTAACATCCTTTTTGAAGTTGTTCAATCCCATAACTC
GAAGAACTAGTCGCCATCTTTCTTCGTTGGTTAGTTCGTGAATGCATTTTAACGCTATTC
TTTTGCCTTTTATACATTGGATTGGTGCATGTATGTACAATTTAAAGTTTTTTTATCTGT
CGTCATATACTTTTAAGTAAAAAATGTAATTACAACTACATGCATATACGACGGTACGTG
TAATCGTATCGTGGTTAAAACGTTT
   
Sequence Name 26-K106772-0022-809-G04C-34ai
GenBank Accession LN497947 [GenBank]
Sequencing primer gene specific primer, 34ai: 5'-GAAACGTTTAAACCACGATACGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K106772-0022-809-G04C-34ai
GCATGTGTTGTATTCATTTTTTACTTAAAAGTATATGACGACAGATAAAAAAACTTTAAA
TTGTACATACATGCACCAATCCAATGTATAAAAGGCAAAAGAATAGCGTTAAAATGCATT
CACGAACTAACCAACGAAGAAAGATGGCGACTAGTTCTTCGAGTTATGGGATTGAACAAC
TTCAAAAAGGATGTTACTATGAGTGGATGAGTGTGCAGGCTAAACACATAGTTGATCTCA
AAGAAGCACTCATGAGTCATCGATCCAAAGAAGATCACAAGCTTGAAGAACTCGTTGGTA
AAATCGGGTGCAAAGCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11