Sequences from confirmation amplicon


Confirmation sequences for line 810C10 and hit At1g72140 are shown.
Line ID 810C10
Locus At1g72140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27142733
Primers used for PCR DA30 - 8474, show primer details
   
Sequence Name 38-K106288-0022-810-C10C-8409
GenBank Accession LN502988 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K106288-0022-810-C10C-8409
TTTCGCACGATTCCTTCTCTTTGTAATGATCAAGAAACACGTGAGTCGTGCGTCTCTCAG
GTCAAAGTAATAATCTTCTTTTGTGCTCTCTACCTAATTGCTTTGGGAGAAGGAGGCTTC
AAGGTATGTCTTCGAGCCTTTGGAGCAGATCAGTTTGATGAACAAGACCCAAATGAGTCC
AAAGCCAAGAGTTCTTACTTTAACTGGTTGTATTTTGCTATATCAATCGGCATATTGACC
ACTCGTCTGGTCACAAATTATGTCCAAGAGAATCTGAGCTGGGCTTTAGGATATGCGATT
CCATGTCTTTCTATGATGCTTGCTCTATTCTTGTTCTTACTTGGAATCAAAACTTACCGC
TTCAGCACCGGAGGAGAAGGAAGACAGGGCAAAAAACATGATAACCCTTTTGTTAGAATT
GGCCGTGTTTTTGTGGCTGCAGCTAGAAACCGGCGACAAACTCCTTCTGATACTTGTCTT
CTTCTGCCTAACGAAAGCACCAAGAAATTCAGGTATTATATGTTTTGTTTGTTTGGTCTT
TTGTTTTCTTTTGTCTTTACACTGTGTTTTTGTTTACAAACAAAATCATTTTTTAGTCAA
GTTTCTTATCTGAACTTAGGTAATTAGTAATTTTACATATATTTTTTAATAGTCCTCCAA
TAGTTAAAAAGTTGTAAATACATAAATGAAAATACAAAATTCTTGAATCATCTAACTAAA
AAAAGGTTGGAACGACTTATAATCTATAGAATTTAAAGCATGTGTTAGCTTCGATAATTT
A
   
Sequence Name 38-K106289-0022-810-C10C-DA30
GenBank Accession LN502989 [GenBank]
Sequencing primer gene specific primer, DA30: 5'-CAAAAGCATGCATGTAAATTATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K106289-0022-810-C10C-DA30
CTTTATCTATGATATAAGTCGTTCCACCTTTTTTTAGTTAGATGATTCAAGAATTTTGTA
TTTTCATTTATGTATTTACAACTTTTTAACTATTGGAGGACTATTAAAAAATATATGTAA
AATTACTAATTACCTAAGTTCAGATAAGAAACTTGACTAAAAAATGATTTTGTTTGTAAA
CAAAAACACAGTGTAAAGACAAAAGAAAACAAAAGACCAAACAAACAAAACATATAATAC
CTGAATTTCTTGGTGCTTTCGTTAGGCAGAAGAAGACAAGTATCAGAAGGAGTTTGTCGC
CGGTTTCTAGCTGCAGCCACAAAAACACGGCCAATTCTAACAAAAGGGTTATCATGTTTT
TTGCCCTGTCTTCCTTCTCCTCCGGTGCTGAAGCGGTAAGTTTTGATTCCAAGTAAGAAC
AAGAATAGAGCAAGCATCATAGAAAGACATGGAATCGCATATCCTAAAGCCCAGCTCAGA
TTCTCTTGGACATAATTTGTGACCAGACGAGTGGTCAATATGCCGATTGATATAGCAAAA
TACAACCAGTTAAAGTAAGAACTCTTGGCTTTGGACTCATTTGGGTCTTGTTCATCAAAC
TGATCTGCTCCAAAGGCTCGAAGACATACCTTGAAGCCTCCTTCTCCCAAAGCAATTAGG
TAGAGAGCACAAAAGAAGATTATTACTTTGACCTGAGAGACGCACGACTCACGTGTTTCT
TGATCATTACAAAGAGAAGGAATCGTTGCTGAAAACGTAAGTCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAG
Primers used for PCR 07au - 8474, show primer details
   
Sequence Name 71-K118446-0022-810-C10C-07au
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 07au: 5'-TCTTAGGCTACACATCCATTTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118446-0022-810-C10C-07au
GATCAAAAAAAAAATTGGATTAAAATTTTAAGGATATTGGCAACTAACCCCCCATTCCAA
TTTGAAACAAAACCCATCCGGGCATCCCAAACTATCCAAATAATTATATGGGTTTAAATT
GGAAACCTTTTTGGTTCCTCCTCCAAAAACCAAAGGGGAACCAAAATCTGGAAAATCTTT
GACCAAAAAAAAACCCCGGGATATTTTGGAAAAACCCTTAAAAAAAATTTAGGGGAGGGG
ACCCGAAATTTTAGAAAAGGGACCACCATGGAGTATGAGGGCCAATTGGGAAAAAAAAAA
AAAGTATTTCCCATTTTTTTTCCATTCCAAAAAGGTAAATTCCCCCCCGGTTTTAAAAAA
   
Sequence Name 31-K118445-0022-810-C10C-8409
GenBank Accession LN500344 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118445-0022-810-C10C-8409
ATAAATTATATTAAAGGTTTTTCCAAACTATCCCNGCGTTTTTTATTAGGTCAAAGATTT
TCAAGATCTTGATTCACATTTGATTTTTGTAGTAGAAACAAAAAAGTATCCAAATTAAAA
CCTATATAATAATATGAATAGTTTGGTATGCACGTATGGTTTCTGTTTCAATTTGGATTG
GGGGTTTAGTCGCATATATCATTAAATTTTTAATCAATTTTTTTTTTTTTGATTCAAATT
CTTATCTGGTTTCTGGGGAATAGAAAAAGGGATGTTTACCCAAAAAA

Last Updated on 06.12.2018 16:11