Sequences from confirmation amplicon


Confirmation sequences for line 811A11 and hit At2g29810 are shown.
Line ID 811A11
Locus At2g29810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12726990
Primers used for PCR GA40 - 8474, show primer details
   
Sequence Name 58-K101385-0022-811-A11C-8409
GenBank Accession LN509654 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K101385-0022-811-A11C-8409
GCCCTTACATTGAATATCCAACACATGAGGTATAAAGAACCGGCTCTGTGAAGCCTAGGC
GCGAGCGTGTTTGGAAGAGTTCCGGTGAAGAGATTAGGTCACGATAGGCTTTGGAGATGA
GAGATAGCTTCGGGTAGTGATATCTCCGGACGAGTGCGACGATGATTACGATGAGTTCTT
CTGGAAGTTCTTTAGGAATCGGAGGAATATTCTCTTCTTCTTCTTGAGGTTTCTTGTTTG
CAGCGCCGCCATTAGAGCCCTCGTCGGAAGTTTCAGAGATTACAGCCATCGCCGTGTGAC
GTCAGCAGCGAATTAACGTAAAGCGAGAGCCTAATCCGAGTTTTGAATAAAAGAAACAAT
AATATGATAACTGAGAGTATTAAATCAGTTTTTATACAATAATGTATTTCTATAAGTACT
TCTCATATTTTCAATGTTTTAGGGATCAATAAACAATTAACGAGTTTCTTCAAAAAT
   
Sequence Name 58-K101391-0022-811-A11C-GA40
GenBank Accession LN509655 [GenBank]
Sequencing primer gene specific primer, GA40: 5'-AATTGCATTTTTGAAGAAACTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K101391-0022-811-A11C-GA40
TCTAACATTGAAATGAGAAGTACTTATAGAAATACATTATTGTATAAAAACTGCTTTAAT
ACTCTCAGTTATCATATTATTGTTTCTTTTATTCAAAACTCGGATTAGGCTCTCGCTTTA
CGTTAATTCGCTGCTGACGTCACACGGCGATGGCTGTAATCTCTGAAACTTCCGACGAGG
GCTCTAATGGCGGCGCTGCAAACAAGAAACCTCAAGAAGAAGAAGAGAATATTCCTCCGA
TTCCTAAAGAACTTCCAGAAGAACTCATCGTAATCATCGTCGCACTCGTCCGGAGATATC
ACTACCCGAAGCTATCTCTCATCTCCNAAGCCTATCGTGACCTAATCTCTTCACCGGAAC
TCTTCCAAACACGCTCGCGCCTAGGCTTCACCGAACCGGTTCTTTATACCTCAGGTGTTG
GTATATTCAATTGTAAGTGGCTTCATGTCCGGGAAATCTACATGGATCAGNCATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCATTTTTTTT

Last Updated on 06.12.2018 16:11