Sequences from confirmation amplicon


Confirmation sequences for line 811C03 and hit At1g20080 are shown.
Line ID 811C03
Locus At1g20080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6963740
Primers used for PCR KU89 - 8474, show primer details
   
Sequence Name 11-K103431-0022-811-C03C-KU89
GenBank Accession LN491075 [GenBank]
Sequencing primer gene specific primer, KU89: 5'-TGGGTTAAAAGCTACTGTTCAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K103431-0022-811-C03C-KU89
CAGAAATAGTAATTTTTGTCTGATTACTTGCAAGGTGGTATCATCAGGTGATTGATTTGC
AAGTTTACGCTACTCCAAGGATTACTCTGAAGCCTTTGGTTCCATCATTCCCCTGTTTTG
CCAACATATTTGTCTCTCTTATGGATAAGGTATAATTCAAACCGGAGCAACCTTATTTTG
TATAATACCTTAAAACTCAAGAGAGTCTACAATGTGATATCTTCTGCAGCCACAAGTCGA
TTTTGGACTGAAGTTACTAGGTGCAGATGTAATGGCGATTCCTGGCTTATACCGATTTGT
CCAGGTAAAGACGTTTTGGAAGTTGTTCCTTTAACTTCCCATGTAAGGAAACATTCTTGT
TCGGACCTGAAACTCTGTTTCTTGTGTTATTGTTTATTACTATTGTAGGAAATCATTAAA
GATCAGGTTGCAAACATGTACCTATGGCCAAAGACTTTGAATGTTCAAATAATGGATCCT
TCTAAGTAAGTTCTTTTTTTTTCTTGTCAATTGACATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 27-K103432-0022-811-C03C-8409
GenBank Accession LN498346 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K103432-0022-811-C03C-8409
GCCATTTACATTGAATATGTCAATTGACAAGAAAAAAAAAGAACTTACTTAGAAGGATCC
ATTATTTGAACATTCAAAGTCTTTGGCCATAGGTACATGTTTGCAACCTGATCTTTAATG
ATTTCCTACAATAGTAATAAACAATAACACAAGAAACAGAGTTTCAGGTCCGAACAAGAA
TGTTTCCTTACATGGGAAGTTAAAGGAACAACTTCCAAAACGTCTTTACCTGGACAAATC
GGTATAAGCCAGGAATCGCCATTACATCTGCACCTAGTAACTTCAGTCCAAAATCGACTT
GTGGCTGCAGAAGATATCACATTGTAGACTCTCTTGAGTTTTAAGGTATTATACAAAATA
AGGTTGCTCCGGTTTGAATTATACCTTATCCATAAGAGAGACAAATATGTTGGCAAAACA
GGGGAATGATGGAACCAAAGGCTTCAGAGTAATCCTTGGAGTAGCGTAAACTTGCAAATC
AATCACCTGATGATACCACCTTGCAAGTAATCAGACAAAAATTACTTATTTTCTTGCGAT
GCAGCGATCAAAAACTTACCTGAACAGTAGCTTTTAACCC

Last Updated on 06.12.2018 16:11