Sequences from confirmation amplicon


Confirmation sequences for line 811H03 and hit At5g22540 are shown.
Line ID 811H03
Locus At5g22540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7482264
Primers used for PCR 48xc - 8474, show primer details
   
Sequence Name 82-K119830-0022-811-H03C-48xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 48xc: 5'-GACACAGTTTAAAAAGATCGAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K119830-0022-811-H03C-48xc
CCATTACTATACTAATGTCTAAGATTGAGTCTGTGTTTTTCCTCGGTTTGAATTTGATTC
CCCGAAGATGAAGCTTCTTGGCAGAGAGAACAAATCCGAGATATTCATGATCATTGAATG
ACGATTTACTGCTATGATCTTTGATTCTTCTTTGAGACGGAACAGGAACAAAAGTCTTAC
GTATCAAATCAAGAAGATGTTTTGCTTCAAGACCATAATGTTTTTCCCAAAAGGTCTCTG
GTTTTTGTAATGCGATTATAAACGACAATCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCAGCACGTTATT
   
Sequence Name 58-K119829-0022-811-H03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K119829-0022-811-H03C-8409
TTTCATTGAATATATCCTGATTGTCGTTTATAATCGCATTACAAAAACCAGAGACCTTTT
GGGAAAAACATTATGGTCTTGAAGCAAAACATCTTCTTGATTTGATACGTAAGACTTTTG
TTCCTGTTCCGTCTCAAAGAAGAATCAAAGATCATAGCAGTAAATCGTCATTCAATGATC
ATGAATATCTCGGATTTGTTCTCTCTGCCAAGAAGCTTCATCTTCGGGGAATCAAATTCA
AACCGAGGAAAAACACAGACTCAATCTTAGACATTAGTTATAGTAATGGTGTGCTTCACA
TTCCTCCGGTAGTCATGGATGATTTTACCGCCTCGATCTTTTTAAACTGTGT

Last Updated on 06.12.2018 16:11