Sequences from confirmation amplicon


Confirmation sequences for line 812F02 and hit At4g29450 are shown.
Line ID 812F02
Locus At4g29450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14482866
Primers used for PCR EU75 - 8474, show primer details
   
Sequence Name 37-K103847-0022-812-F02C-EU75
GenBank Accession LN502591 [GenBank]
Sequencing primer gene specific primer, EU75: 5'-CGACCAGAAGTTCACGTTTACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K103847-0022-812-F02C-EU75
GCAGCTTTTTACCTCGTAGTTTCCATACATAAAGGAAGCTCTAATGAGATAAAGATGATC
TTTTCCTTGTGAAAGTTTTAAGGTGTAACAGTTTCTGTTTCCTTGAGGGAAAGCTCTTAC
TTCAGCAAGAGTTGACAGCAATACCGGGTTTTTTGGATAGCCGTATTCTTCAGAGACCTT
GAAGTTTACGCCGGTGGTTATGAAAGCTTCATCGGAGACGTAATTAATGTTAGTCATAGT
GTCTTCAGGGGTATCGTAAGGAGGTATGCCACAATCGATGCTGATGTAACCTGGATCAGA
GGAGAAGAAAGGGGAGTTACTTCCAACACAAGTAAAATTGTTTATGACGTCTGAGAACGT
TCATGGATAGGTTTAAAAATCTGCCTAACTTCGTTTCTCTATATTGACAAAAACGTAGAT
NACAACTTTCAAGAACCTTCAAGAACATCGGTCAATTTCAAATTAGAAAACAGAGAANAG
AGAGAATTGTATGATTACTTGTAACACAAGACATTGTATAAGGGTTTGACTAANAATCCA
AGACATGTTTAAATGTATTAGAAGTTACATTTTAAATGTATCACATGTTTAAATACATGT
TTATATATNTTTCACATCTGGATCATCTTTAAATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCANAAATGTAGA
   
Sequence Name 53-K103848-0022-812-F02C-8409
GenBank Accession LN508133 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K103848-0022-812-F02C-8409
CCATTTACATTGAATATATTTAAAGATGATCAAGATGTGAAATATATATAAACATGTATT
TAAACATGTGATACATTTAAAATGTAACTTCTAATACATTTAAACATGTCTTGGATTTTT
AGTCAAACCCTTATACAATGTCTTGTGTTACAAGTAATCATACAATTCTCTCTTTTCTCT
GTTTTCTAATTTGAAATTGACCGATGTTCTTGAAGGTTCTTGAAAGTTGTGATCTACGTT
TTTGTCAATATAGAGAAACGAAGTTAGGCAGATTTTTAAACCTATCCATGAACGTTCTCA
GACGTCATAAACAATTTTACTTGTGTTGGAAGTAACTCCCCTTTCTTCTCCTCTGATCCA
GGTTACATCAGCATCGATTGTGGCATACCTCCTTACGATACCCCTGAAGACACTATGACT
AACATTAATTACGTCTCCGATGAAGCTTTCATAACCACCGGCGTAAACTTCAAGGTCTCT
GAAGAATACGGCTATCCAAAAAACCCGGTATTGCTGTCAACTCTNGCTGAAGTAAGAGCT
TTCCCTCAAGGAAACAGAAACTGTTACACCTTAAAACTTTCACAAGGAAAAGATCATCTT
TATCTCANTAGAGCTTCCTTTATGTATGGAAACTACAATGGTAAAAAAGCTTTGCCTGAG
TTTGATCTCTATGTAAACG
Primers used for PCR BB84 - 3144, show primer details
   
Sequence Name 66-K106975-0022-812-F02C-3144
GenBank Accession LN511620 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K106975-0022-812-F02C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTAATCAACTGGATTTTATTAGA
AGACAACATGTATCTCTGCTTCTTGACTTTAGTGAAGTTATTAAAGATGTGATTAACATC
CAAAATAATAGATAAAATACATAGACTGAGTCATTGATTGCCAGTTGATTTTGATCTGGN
GACAGAATA
   
Sequence Name 82-K106977-0022-812-F02C-BB84
GenBank Accession LN514197 [GenBank]
Sequencing primer gene specific primer, BB84: 5'-ATTCTGTCCCAGATCAAAATCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K106977-0022-812-F02C-BB84
TATTTATCTATTATTTTGGATGTTAATCACATCTTTAATAACTTCACTAAAGTCAAGAAG
CAGAGATACATGTTGTCTTCTAATAAAATCCAGTTGATTAGTTTAAACCGAAGGCGGGAA
ACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAG
AGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
CACATCAATCCA
Primers used for PCR 65wl - 3144, show primer details
   
Sequence Name 71-K118414-0022-812-F02C-65wl
GenBank Accession LN512632 [GenBank]
Sequencing primer gene specific primer, 65wl: 5'-GCCCTTATTCGATAGTGATACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118414-0022-812-F02C-65wl
CATTTCTTCGTGGGATAGGTATTATTGAGCTCTGATTTTGTGACATTCAATATAAGCTAG
TACTAGTGATGAAACCACATGACTCTTTTTCTGTTACATTGTGACATTTCTTATGATAAG
ATATATGTGAATAATCTCTGCGCAGGTAATATATTTAATCCCCCGGTGCTGGGAAGCGTG
CCCGTCGTGGAGGTGGATCTCGCTCGGAGTAAAGTGGACACAAAGGATGGCCTTGGAACA
AAACTAGGCCTTCAAACAAAAGAAGGCTCCAAAGTCTCTGCTTCTTTAAATATATGAGCC
CTTCTAGTACTATTTCTTGATCATCTTCTAAAGTGACTACTCTCTGTAACTTCGTTGTTA
TAGAAAATTCATAAAACAAAAAAAGACTTTGAATTCTGTCCCAGATCAAAATCAACTGGC
AATCAATGACTCAGTCTATGTATTTTATCTATTATTTTGGATGTTAATCACATCTTTAAT
AACTTCACTAAAGTCAAGAAGCAGAGATACATGTTGTCTTCTAATAAAATCCAGTTGATT
AGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAAT
GAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCAT
CCCTTACGTCAGTGGAGATAT
   
Sequence Name 23-K118413-0022-812-F02C-35St
GenBank Accession LN496756 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118413-0022-812-F02C-35St
TCCACTATCCTTGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAG
GGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTAATCAACTGGATTTT
ATTAGAAGACAACATGTATCTCTGCTTCTTGACTTTAGTGAAGTTATTAAAGATGTGATT
AACATCCAAAATAATAGATAAAATACATAGACTGAGTCATTGATTGCCAGTTGATTTTGA
TCTGGGACAGAATTCAAAGTCTTTTTTTGTTTTATGAATTTTCTATAACAACGAAGTTAC
AGAGAGTAGTCACTTTAGAAGATGATCAAGAAATAGTACTAGAAGGGCTCATATATTTAA
AGAAGCAGAGACTTTGGAGCCTTCTTTTGTTTGAAGGCCTAGTTTTGTTCCAAGGCCATC
CTTTGTGTCCACTTTACTCCGAGCGAGATCCACCTCCACGACGGGCACGCTTCCCAGCAC
CGGGGGATTAAATATATTACCTGCGCAGAGATTATTCACATATATCTTATCATAAGAAAT
GTCACAATGTAACAGAAAAAGAGTCATGTGGTTTCATCACTAGTACTAGCTTATATTGAA
TGTCACAAAATCAGAGCTCAATAATTACCTATCCCACTGAAGAAAATGCCGTAATCCATT
CCATTGAACACGGTTGGTATCA

Last Updated on 06.12.2018 16:11