Sequences from confirmation amplicon


Confirmation sequences for line 813G10 and hit At4g08500 are shown.
Line ID 813G10
Locus At4g08500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5405859
Primers used for PCR HM15 - 8474, show primer details
   
Sequence Name 41-K029126-022-813-G10C-8409
GenBank Accession LN503956 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K029126-022-813-G10C-8409
AGATTTCCCGGACAGTAGCCGGCTGACAAATGTAGTCGGCTAATGGATGTTTATGTTATA
TGTAGAGATGGGGACTTCTTTGCTGTCAAGGAAGTTTCACTTCTTGATCAGGGAAGTCAG
GCACAAGAATGCATACAACAACTTGAGGGGGTATGGAAATGGAAGTTTCATATACATGGG
TCAATGATCCGTAGCATTTATCACTTCTGTCATTATCTCATGGAGCAGTCAAAAGTAGGT
TGCTAATCCACCTTACCTTTGCCATATTTTTCATTTTCTACAGGAGATTAAACTACTTAG
TCAGCTTCAGCATCAGAATATTGTGAGATATCGTGGCACAGCCAAGGTGTGGTCCTGTTC
TCTCTTTCTTTTTCTTAGTTGCAGTTGCCTAACTATTTCATTGTGCAATAATAACTCATT
TCGCTGTTTTCAGGATGGGTCAAACTTGTACATTTTTCTTGAGCTTGTAACCCAAGGGTC
CCTTCTAAAACTCTACCAAAGATACCAGCTTCGGGACTCTGTAGTCTCCTTGTACACTAG
ACAGATTCTTGACGGTTTGAAATATCTCCACGATAAAGGTTTTATTCACAGGTTAGACTA
GTAAACTCTCTTGGTACAGCTGTAATTCCCAAGCATCTATCTAATTTCCTGCATCTCANG
GGTTACTTATTTTTATCATTTTATGCAACAAAGGGGACATCAAATG
   
Sequence Name 42-K029126-022-813-G10C-HM15
GenBank Accession LN504339 [GenBank]
Sequencing primer gene specific primer, HM15: 5'-GGGTACTAAACGATGGACCAAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K029126-022-813-G10C-HM15
ATTACTTTAGAGCAAAGCTATAAATATCTATTTATGGAAAAAAACCTTTGCCAATCCAAA
ATCTGCAAGTTTGACGGCGCCATTAGCGTCCACCAATATATTTGCACATTTGATGTCCCT
TTGTTGCATAAAATGATAAAAATAAGTAACCCATGAGATGCAGGAAATTAGATAGATGCT
TGGGAATTACAGCTGTACCAAGAGAGTTTACTAGTCTAACCTGTGAATAAAACCTTTATC
GTGGAGATATTTCAAACCGTCAAGAATCTGTCTAGTGTACAAGGAGACTACAGAGTCCCG
AAGCTGGTATCTTTGGTAGAGTTTTAGAAGGGACCCTTGGGTTACAAGCTCAAGAAAAAT
GTACAAGTTTGACCCATCCTGAAAACAGCGAAATGAGTTATTATTGCACAATGAAATAGT
TAGGCAACTGCAACTAAGAAAAAGAAAGAGAGAACAGGACCACACCTTGGCTGTGCCACG
ATATCTCACAATATTCTGATGCTGAAGCTGACTAAGTAGTTTAATCTCCTGTAGAAAATG
AAAAATATGGCAAAGGTAAGGTGGATTAGCAACCTACTTTTGACTGCTCCATGAGATAAT
GACAGAAGTGATAAATGCTACGGATC

Last Updated on 06.12.2018 16:11