Sequences from confirmation amplicon


Confirmation sequences for line 814B04 and hit At3g07880 are shown.
Line ID 814B04
Locus At3g07880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2515316
Primers used for PCR HP81 - 8474, show primer details
   
Sequence Name 70-K033720-022-814-B04C-8409
GenBank Accession LN512321 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K033720-022-814-B04C-8409
TTACAATTGAATATATCCTGGCATCACGTGGTTGTATGGCTCCAACTGAGGACTAAAGGT
TCCAAGCATTTCCTTAGCTCTGTCCACTGTAGCCATTAAAGAAAGGAATTGGATCACATT
GTCTCTCAACCATCGAGAGGACCAATTAATTAAAAACAAACTTGTGGTCTATTGTTTTAC
CTTTGACACCGGTCTTCCAAACTGTATTGGTGTACCTAAGACCAGAGACAATGTTGTTGT
TAACATGAAAAGTGAATTTCAAGTTGTACTTGCTCCCTTCTTTCAAAGTAAACCACATGC
CCTTTGGATTTCCATTCTCAGGAACCAAGAGAACAATGTCGGGTCTTCCAGGAGATATGA
TGGCGAGGCTATCAATCCTCACTTCCGGATCAAGAGTCTCTGCGTATCAAAAGAGATATA
AGGTGACAAATGCATAAATGCAAAAAACACATGTTCCAATCTTAAGTTGATATCTCTACA
GTAGCACTCAGAAAAATGGAAAATCAAGCAAAGAAATAAACATAGACACCAAATTCCGGA
ATCATGGGAAAAAAAATTGT
   
Sequence Name 32-K033720-022-814-B04C-HP81
GenBank Accession LN500483 [GenBank]
Sequencing primer gene specific primer, HP81: 5'-TACAATTTTCTTTCCCATGATTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K033720-022-814-B04C-HP81
TTGCTTGATTTTCCATTTTTCTGAGTGCTACTGTAGAGATATCAACCTCCTATAGGAACA
TGTGTTTTTTGCATTTATGCATTTGTCTCTCTAAAACTCTTTTGATACGCAGAGACTCTT
GATCCGGAAGTGAGGATTGATAGCCTCGCCATCATATCTCCTGGAAGACCCGACATTGTT
CTCTTGGTTCCTGAGAATGGAAATCCAAAGGGCATGTGGTTTACTTTGAAAGAAGGGAGC
AAGTACAACTTGAAATTCACTTTTCATGTTAACAACAACATTGTCTCTGGTCTTAGGTAC
ACCAATACAGTTTGGAAGACCGGTGTCAAAGGTAAAACAATAGACCACAAGTTTGTTTTT
AATTAATTGGTCCTCTCGATGGTTGAGAGACAATGTGATCCAATTCCTTTCTTTAATGGC
TACAGTGGACAGAGCTAAGGAAATGCTTGGAACCTTTAGTCCTCAGTTGGAGCCATACAA
CCACGTGATGCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11