Sequences from confirmation amplicon


Confirmation sequences for line 814D03 and hit At1g07670 are shown.
Line ID 814D03
Locus At1g07670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2370309
Primers used for PCR CN11 - 8474, show primer details
   
Sequence Name 67-K031140-022-814-D03C-CN11
GenBank Accession LN511730 [GenBank]
Sequencing primer gene specific primer, CN11: 5'-CCATTGAAATGTTCAACTCTCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K031140-022-814-D03C-CN11
ATGCCCCCTGGGTTAACCCGTGGCTGCTCCTGGCAATGGCTGTCTCATTTGGACTCCACT
TTGTGATATTGTACGTCCCATTCTTGGCTCAGGTGTTTGGTATTGTGCCACTGAGCTTGA
ACGAGTGGCTGCTAGTGCTGGCTGTGTCGCTGCCGGTAATTTTGATAGACGAAGTGCTCA
AGTTTGTAGGAAGGTGCACGAGTGGCTACCGCTACTCACCTCGCACTCCCTCAGCTAAGC
AAAAGGAGGAAAGACAATCTACATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 32-K031140-022-814-D03C-8409
GenBank Accession LN500461 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K031140-022-814-D03C-8409
GAGCCATTTACAATTGAATATATCATGTAGATTGTCTTTCCTCCTTTTGCTTAGCTGAGG
GAGTGCGAGGTGAGTAGCGGTAGCCACTCGTGCACCTTCCTACAAACTTGAGCACTTCGT
CTATCAAAATTACCGGCAGCGACACAGCCAGCACTAGCAGCCACTCGTTCAAGCTCAGTG
GCACAATACCAAACACCTGAGCCAAGAATGGGACGTACAATATCACAAAGTGGAGTCCAA
ATGAGACAGCCATTGCCAGGAGCAGCCACGGGTTAACCCAGGGTGGCATCGTCACCAGGC
TGCCATCTTCTGAGAGTGCATTCAGAGAGTTGAACATNTTCAATGG

Last Updated on 06.12.2018 16:11