Sequences from confirmation amplicon


Confirmation sequences for line 814G02 and hit At2g28400 are shown.
Line ID 814G02
Locus At2g28400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12149115
Primers used for PCR 15ms - 8474, show primer details
   
Sequence Name 14-K114626-0022-814-G02C-15ms
GenBank Accession LN492611 [GenBank]
Sequencing primer gene specific primer, 15ms: 5'-GATCCGGTATGCTTGCTTAGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114626-0022-814-G02C-15ms
CGGTGTCTGAGAAAAAAAAATCTGGTTTGTTAATAAATTCCATCCATGTAAGAGTTATTA
TTGTTTTCCAATTCATCTTCCTACGAAATTTCGAAGAAATATAATAAATTGCGTTCTCTT
CTTTAACACATGATTCAAATATTCACAGCGTAGTTAATAATTGAATAAACCACAAGTTAA
AATAAAATAACCTTTTAATCGGTTGCCGTGTAGAGCCCTTGACAGTCATCGTTTCGTTTG
GATAAAATCGCAAACTTCTACAAAATTTCCAAGGGCATTTCCGTAATTTCGTAGAAAACG
GTGACCAAAAGAAATTAACACGAGGAGGTTGAAGTCTACATGAAGATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATTTCCG
   
Sequence Name 14-K114625-0022-814-G02C-8409
GenBank Accession LN492610 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114625-0022-814-G02C-8409
TACATTGAATCTTCTGTAGACTTCAACCTCCTCGTGTTAATTTCTTTTGGTCACCGTTTT
CTACGAAATTACGGAAATGCCCTTGGAAATTTCGTAGAAGTTTGCGATTTTATCCAAACG
AAACGATGACTGTCAAGGGCTCTACACGGCAACCGATTAAAAGGTTATTTTATTTTAACT
TGTGGTTTATTCAATTATTAACTACGCTGTGAATATTTGAATCATGTGTTAAAGAAGAGA
ACGCAATTTATTATATTTCTTCGAAATTTCGTAGGAAGATGAATTGGAAAACAATAATAA
CTCTTACATGGATGGAATTTATTAACAAACCAGATTTTTTTTTTCTCAAACACCGTTTTC
CTCCTTTGACAATGGTATTAGTTATAAAGTATTAGTATAATCTAAGC

Last Updated on 06.12.2018 16:11