Sequences from confirmation amplicon


Confirmation sequences for line 815B08 and hit At4g16150 are shown.
Line ID 815B08
Locus At4g16150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9149746
Primers used for PCR MK18 - 8474, show primer details
   
Sequence Name 51-K030903-022-815-B08C-8409
GenBank Accession LN507395 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K030903-022-815-B08C-8409
CATTTACAATTGAATATATTCATTATGAACTGTATTACAAGTATTATGGACACCAGAACT
GGTATCTTCAGCTACAATTTTAGGAGATAAGTGGTCAGTGATCGAACTTGAGTACGAGTT
CCCAGGTGTTGCTGGAGCTGCATGAACCTGTTTAACCAAAATTTCTCAGAGGGCAACAAA
ATGATATTATCTTCGTAACTAAAATCTGCCAGAAGAAGGCTGGACTCTATGCGACAACGA
AAATACATCATCCTAATCCATAACCACCTCTAACATACATATTATATTCTGGGGACAGGT
AGATAACAAACCTCATGTGTCTCGCGATAGTGGACAAGAACTATGTGCTCCTGAGACCTG
CCATTATAAGAAGAGGTGATTTATTTTCATAACCTTCAGAAACAAAGCAAAGGAAAGAAG
GCATCTAATAAACTATTTCCTCAAAGGATAAACCAATAATCGTTTGTTGACACATACTTA
TCCAATAAC
   
Sequence Name 52-K030903-022-815-B08C-MK18
GenBank Accession LN507707 [GenBank]
Sequencing primer gene specific primer, MK18: 5'-CGAAGGTGTTACTGGTTATTGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K030903-022-815-B08C-MK18
GTTATTAGATGCCTTCTTTCCTTTGCTTCGCTTCACGAGGTTATGAAAATAAATCACCTC
TTCTTATAATGGCAGGTCTCAGGAGCACATAGTTCTTGTCCACTATCGCGAGACACATGA
GGTTTGTTATCTACCTGTCCCCAGAATATAATATGTATGTTAGAGGTGGTTATGGATTAG
GATGATGTATTTTCGTTGTCGCATAGAGTCCAGCCTTCTTCTGGCAGATTTTAGTTACGA
AGATAATATCATTTTGTTGCCCTCTGAGAAATTTTGGTTAAACAGGTTCATGCAGCTCCA
GCAACACCTGGGAACTCGTACTCAAGTTCGATCACTGACCACTTATCTCCTAAAATTGTA
GCTGAAGATACCAGTTCTGGTGTCCATAATACTTGTAATACAGTTCATAATGAATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTA

Last Updated on 06.12.2018 16:11