Sequences from confirmation amplicon


Confirmation sequences for line 815C08 and hit At1g48360 are shown.
Line ID 815C08
Locus At1g48360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17872492
Primers used for PCR KU09 - 8474, show primer details
   
Sequence Name 76-K102435-0022-815-C08C-8409
GenBank Accession LN513373 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K102435-0022-815-C08C-8409
TTACATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCATAAGGG
GAACAGAAAACAGAATGAGAGTTTTCATTTGCGTCATATGAGACAACTATTGAGTTTCAT
TCACCTGGAATCGGGTTTGGAAAACGCCAGGGACATCGGAAAATAGAATATCCCACATGA
GAAGCCCGAAAATCGTTAACCAGATACTACTCTCTGTATGAATTCCTTGCCATCCTCCTC
CTTCTCCACTGTAATACTGCAATGCGAGCTGCTCCACTCCACATTGTTCTCCATCTTCTC
CATAAAACCTATTTTTTATGCCAACTTCACAGTTCAGTGATCTCCCTTGAATAGTAACCT
GAAATGCAAAATTGCACATTTGAGGACAAAGTAAGAAGATGAACAACTTTATATTTTATC
TTTTCACTTTGAATAGAAGTATAACCTCGGGGATCTTGTTGTCGACGAGATTTGAAAAAG
TGGGAGTTTTCCAGCGTCTTGGAGGTTTTGCCAAACGAAGAATCCGCCTTTGCAAAGCTA
CCCTTGAACCAGCACGCACCCAAGGATCCAATAGTCCCTGCTCTGCAACTGTAAGACTCT
CATTTGGACGTCCCATGTGCTCCAAATCCGTTGATAACCTGATTGTCCAGT
   
Sequence Name 76-K102436-0022-815-C08C-KU09
GenBank Accession LN513374 [GenBank]
Sequencing primer gene specific primer, KU09: 5'-TACTGGACAGTCAGGTTATCAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K102436-0022-815-C08C-KU09
GTTGCAGAGCAGGGACTATTGGATCCTTGGGTGCGTGCTGGTTCAAGGGTAGCTTTGCAA
AGGCGGATTCTTCGTTTGGCAAAACCTCCAAGACGCTGGAAAACTCCCACTTTTTCAAAT
CTCGTCGACAACAAGATCCCCGAGGTTATACTTCTATTCAAAGTGAAAAGATAAAATATA
AAGTTGTTCATCTTCTTACTTTGTCCTCAAATGTGCAATTTTGCATTTCAGGTTACTATT
CAAGGGAGATCACTGAACTGTGAAGTTGGCATAAAAAATAGGTTTTATGGAGAAGATGGA
GAACAATGTGGAGTGGAGCAGCTCGCATTGCAGTATTACAGTGGAGAAGGAGGAGGATGG
CAAGGAATTCATACAGAGAGTAGTATCTGGTTAACGATTTTCGGGCTTCTCATGTGGGAT
ATTCTATTTTCCGATGTCCCTGGCGTTTTCCAAACCCGATTCCAGGTGAATGAAACTCAA
TAGTTGTCTCATATGACGCAAATGAAAACTCTCATTCTGTTTTCTGTTCCCCTTATGCAA
GCTCCACCGGGTGCAAAGCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCCAA

Last Updated on 06.12.2018 16:11