Sequences from confirmation amplicon


Confirmation sequences for line 815G05 and hit At1g22640 are shown.
Line ID 815G05
Locus At1g22640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8006976
Primers used for PCR HM17 - 8474, show primer details
   
Sequence Name 15-K028919-022-815-G05C-8409
GenBank Accession LN492836 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K028919-022-815-G05C-8409
CGACGTCGTTCCACTACTCTCACAATTATTAACCATACCAATCTCTTCACGCACCGGTTC
CGGTTTAACCGGTCCAGAGAAATCAAGATGTATAGTCTCAACGACATCGTTTTGAAGAGA
CGACGGAGACACGACGGATTCGTTGATCAGACGGTGAGAGTTTGGATCAATCCCACGGCT
GAGAAGCTTCCTCTTGATATGAGTGTTCCAATAGTTCTTGATCTCGTTATCTGTTCTTCC
TGGTAATCTCCCAGCTATTAAAGACCATCTATAACAACAACACAAAGTTTCAGAATCTTT
CACAAATATAATCATATTCTTAAAAAAGTGTGAGAGAAAGAGACTAACTTGTTACCGAGC
AAGCTATGGAGCTTGATGATGAGTTCATCTTCTTCTTCAGTAAAATTGCCTCTTTTGAGA
TCTGGTCTTAGATAATTCATCCATCTCAATCTACAACTCTTACCACATCTTTGTAATCCT
GCACAAAGTTCAATCAAAAATCAAATCTTTACCTCTCTGTAAATATAAAGAAACAGAGGA
TGTATGAATAAGTACCAGCGGCGCGAGGGAGAGATCGCCAGCAACCTTCACCGTGTTTAC
GGATGTAATCAACAAGAAGCTGATCTTCTTCTTTAGTCCAAGC
   
Sequence Name 16-K028919-022-815-G05C-HM17
GenBank Accession LN493305 [GenBank]
Sequencing primer gene specific primer, HM17: 5'-ATGAACAAAGGAGCTTGGACTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K028919-022-815-G05C-HM17
CGTAACACGGTGAAGGTTGCTGGCGATCTCTCCCTCGCGCCGCTGGTACTTATTCATACA
TCCTCTGTTTCTTTATATTTACAGAGAGGTAAAGATTTGATTTTTGATTGAACTTTGTGC
AGGATTACAAAGATGTGGTAAGAGTTGTAGATTGAGATGGATGAATTATCTAAGACCAGA
TCTCAAAAGAGGCAATTTTACTGAAGAAGAAGATGAACTCATCATCAAGCTCCATAGCTT
GCTCGGTAACAAGTTAGTCTCTTTCTCTCACACTTTTTTAAGAATATGATTATATTTGTG
AAAGATTCTGAAACTTTGTGTTGTTGTTATAGATGGTCTTTAATAGCTGGGAGATTACCA
GGAAGAACAGATAACGAGATCAAGAACTATTGGAACACTCATATCAAGAGGAAGCTTCTC
AGCCGTGGGATTGATCCAAACTCTCACCGTCTGATCAACGAATCCGTCGTGTCTCCGTCG
TCTCTTCAAAACGATGTCGTTGAGACTATACATCTTGATTTCTCTGGACCGGTTAAACCG
GAACCGGTGCGTGAAGAGATTGGTATGGTTAATAATTGTGAGAGTAGTGGAACGACGTCG
GAGAAGGATTATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACCAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT

Last Updated on 06.12.2018 16:11