Sequences from confirmation amplicon


Confirmation sequences for line 815H10 and hit At1g55730 are shown.
Line ID 815H10
Locus At1g55730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20831341
Primers used for PCR MG36 - 8474, show primer details
   
Sequence Name 21-K032923-022-815-H10C-8409
GenBank Accession LN495564 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K032923-022-815-H10C-8409
TTCGGCTTTAGTTTAGGATACAAACTTTTTATGTTCTTTTGGGTTGATTTATATATCATC
TGTCAAAAAATAAGAAGACAAAAACAGGTTTCAACAGAGAGTTGACACAGAGAAAGGAAT
ATATATAGCAAATTCAACACTAACCTTGATGAGGATCTTCGTGTACAAAGAAACTAGCGG
CGACTATCAAATAGCAAAGAATGAGCATCAATCCTTTGAAGTAATTCGATGTTCCTTCCT
GCATAAAAACACCACACACAAAGACATTTAACAACTTTGAAGAGAGAGATATACTCGAGA
TTGGTTTCGTAATTAGCTTCAAGATTTACCTGGAGAAAGAAAGCTACAACAATAACAGTT
ATGAACAGAGTAGCCGTCTCAAATAGCTGGAAATTTAGGTCCATTTGGGCACCCATCATC
CATCCGATAACCACGCAAAAAGGAACCTAAATCCAATCAAACTATCAGACAAAATCTTTT
AGAGAATGTTCAGTACTAGACCCAAGTTCACATTTCATATACATACCGCGAACATAGAAA
TCTGAATGGA
   
Sequence Name 21-K032923-022-815-H10C-MG36
GenBank Accession LN495565 [GenBank]
Sequencing primer gene specific primer, MG36: 5'-TATTGGATCCTCCATTCAGATTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K032923-022-815-H10C-MG36
TTGGGTCTATACTGAACATTCTCTAAAAGATTTTGTCTGATAGTTTGATTGGATTTAGGT
TCCTTTTTGCGTGGTTATCGGATGGATGATGGGTGCCCAAATGGACCTAAATTTCCAGCT
ATTTGAGACGGCTACTCTGTTCATAACTGTTATTGTTGTAGCTTTCTTTCTCCAGGTAAA
TCTTGAAGCTAATTACGAAACCAATCTCGAGTATATCTCTCTCTTCAAAGTTGTTAAATG
TCTTTGTGTGTGGTGTTTTTATGCAGGAAGGAACATCGAATTACTTCAAAGGATTGATGC
TCATTCTTTGCTATTTGATAGTCGCCGCTAGTTTCTTTGTACACGAAGATCCTCATCAAG
GTTAGTGTTGAATTTGCTATATATATTCCTTTCTCTGTGTCAACTCTCTGTTGAAACCTG
TTTTTGTCTTCTTATTTTTTGACAGATGATATATAAATCAACCCAAAAGAACATAAAAAG
TTTGTATCCTAAAACTAAAGCCTGAAGATCTCAGGAACACATCATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11