Sequences from confirmation amplicon


Confirmation sequences for line 816A11 and hit At2g45510 are shown.
Line ID 816A11
Locus At2g45510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18754721
Primers used for PCR 26gd - 8474, show primer details
   
Sequence Name 85-K112063-0022-816-A11C-26gd
GenBank Accession LN514565 [GenBank]
Sequencing primer gene specific primer, 26gd: 5'-GTTATTGGTGTCTTGTTCCACCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K112063-0022-816-A11C-26gd
CACTATAAAAGTATGGAAAGTATTCTGGTTAAGTACTCAAGTGCTTGTCCTCGGGATTGC
ACAGAGATGGAGTCCTCCATCGACATGAAGAGTAAGCATTCTCTTGTAATACACCTTGCT
GTTCTCATCAGCCATTTTGAAGCGAAAGAAGTGAAGAAGTGCCATTGATACTATCTTCAT
CTGCCGGTATGCGAAATCCTTGCCAAGACAGATTCTTGGACCAGCCTACATACATAGCCA
AAGAAATAAAACATCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAACGTTATA
   
Sequence Name 82-K112047-0022-816-A11C-8409
GenBank Accession LN514210 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K112047-0022-816-A11C-8409
TTGAATATATCCTGAGATGTTTTATTTCTTTGGCTATGTATGTAGGCTGGTCCAAGAATC
TGTCTTGGCAAGGATTTCGCATACCGGCAGATGAAGATAGTATCAATGGCACTTCTTCAC
TTCTTTCGCTTCAAAATGGCTGATGAGAACAGCAAGGTGTATTACAAGAGAATGCTTACT
CTTCATGTCGATGGAGGACTCCATCTCTGTGCAATCCCGAGGACAAGCACTTGAGTACTT
AACCAGAATACTTTCCATACTTTTATAGTGCCTCCATGAGTTTTGGTAACATTCTCAATA
AAGGTGGAACAAGACACCAATAA

Last Updated on 06.12.2018 16:11