Sequences from confirmation amplicon


Confirmation sequences for line 817B01 and hit At2g26660 are shown.
Line ID 817B01
Locus At2g26660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11339743
Primers used for PCR KU60 - 8474, show primer details
   
Sequence Name 33-K103355-0022-817-B01C-KU60
GenBank Accession LN500958 [GenBank]
Sequencing primer gene specific primer, KU60: 5'-GGCCAGTTAAACAAATTACAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K103355-0022-817-B01C-KU60
TTACAAGTAACACAAAGATCGTGGAGAATATGACTCTTAACATAGACGTAATGTTAATAC
TAACTCAAGTTCCCTACTAATCAATCTTTCACAAAACTTCTAAAAATATTGCCCATTGAC
ACGGTTTCACTTACTAGGAAATGCTAAAAACTAGAGCTTGACCTTGTAAACGAGTTTTAA
CATCTAAAAACTATCAATTGTCCAACATGTGAGCTTGTTCTGTTTACTCAACGTCGGCTT
TCGGGCTACGAATGATTAGAACATATGAGAAAAGAACGAAAAACAATAACCATGGAGATG
ACATGAGAAAGAAAAGTTTACCTGTATAGTTAAGAGCACTATAATTCATCAACAAAACCA
TTTCTCCATGAAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 49-K103356-0022-817-B01C-8409
GenBank Accession LN506806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K103356-0022-817-B01C-8409
TTTTGTGATGAATATAGTGCTCTTAACTATACAGGTAAACTTTTCTTTCTCATGTCATCT
CCATGGTTATTGTTTTTCGTTCTTTTCTCATATGTTCTAATCATTCGTAGCCCGAAAGCC
GACGTTGAGTAAACAGAACAAGCTCACATGTTGGACAATTGATAGTTTTTAGATGTTAAA
ACTCGTTTACAAGGTCAAGCTCTAGTTTTTAGCATTTCCTAGTAAGTGAAACCGTGTCAA
TGGGCAATATTTTTAGAAGTTTTGTGAAAGATTGATTAGTAGGGAACTTGAGTTAGTATT
AACATTACGTCTATGTTAAGAGTCATATTCTCCACGATCTTTGTGTTACTTGTTAAATAG
AAATGTGCCCACATGAAGGAATATGCTGTAATTTGTTAAACTGGC

Last Updated on 06.12.2018 16:11