Sequences from confirmation amplicon


Confirmation sequences for line 817D04 and hit At4g40030 are shown.
Line ID 817D04
Locus At4g40030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18555884
Primers used for PCR TA22 - 8474, show primer details
   
Sequence Name 37-K030142-022-817-D04C-8409
GenBank Accession LN502499 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K030142-022-817-D04C-8409
CTTTCCGGACATGGGCATTATGGTCACACGCTTGGCATGAATGGCACAAAGGTTAGTGTC
TTCAAAGAGACCAACAAGATATGCTTCTGCAGCTTCCTGGAGAGCTAAGACAGCATGGCT
CTGGAAACGCAAATCAGTCTTGAAATCTTGAGCAATCTCACGGACTAGCCTCTGAAATGG
CAGTTTCCTGATAAGCAACTCAGTACTCTTCTGGTACTTACGGATTTCACTGAACACCAA
AAGATAAGAGGAATGTTAACACACAATGTTACATAATCTCAAAGAATCACATACAAATCG
AAGAAGGGACAAACCGGAGAGCAACAGTTCCAGGACGGTAACGATGTGGTTTCTTGACTC
CACCAGTAGTTGGTGCAGATTTACGAGCAGCCTGAGGTTAATAAAGAAGGAGATTAACAT
TCAACTTCACAACACAAGTATTATTTGAAAAAAGAGACAAGAAGGAACAAAACCTTAGTA
GCAAGTTGCTTCCTTGGAGCTTTACCTCCGGTGGACTTACGAGCGGTTTGCTTGGTACGA
GCCATCTGCGCATTTAACAAGAAATTGAACAGTCAATTGGGGATTTTCATTATCCATAAC
TAAATTTTGAAGAAATTGGAATACTAAACGTCACCACT
   
Sequence Name 37-K030142-022-817-D04C-TA22
GenBank Accession LN502500 [GenBank]
Sequencing primer gene specific primer, TA22: 5'-TGGATTAGGGTTTTAAGTGGTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K030142-022-817-D04C-TA22
TATGGATAATGAAAATCCCCAATTGACTGTTCAATTTCTTGTTAAATGCGCAGATGGCTC
GTACCAAGCAAACCGCTCGTAAGTCCACCGGAGGTAAAGCTCCAAGGAAGCAACTTGCTA
CTAAGGTTTTGTTCCTTCTTGTCTCTTTTTTCAAATAATACTTGTGTTGTGAAGTTGAAT
GTTAATCTCCTTCTTTATTAACCTCAGGCTGCTCGTAAATCTGCACCAACTACTGGTGGA
GTCAAGAAACCACATCGTTACCGTCCTGGAACTGTTGCTCTCCGGTTTGTCCCTTCTTCG
ATTTGTATGTGATTCTTTGAGATTATGTAACATTGTGTGTTAACATTCCTCTTATCTTTT
GGTGTTCAGTGAAATCCGTAAGTACCAGAAGAGTACTGAGTTGCTTATCAGGAAACTGCC
ATTTCAGAGGCTAGTCCGTGAGATTGCTCAAGATTTCAAGACTGATTTGCGTTTCCAGAG
CCATGCTGTCTTAGCTCTCCAGGAAGCTGCAGAAGCATATCTTGTTGGTCTCTTTGAAGA
CACTAACCTTTGTGCCATTCATGCCAAGCGTGTGACCATAATGCCCATGTCCGGGAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGT

Last Updated on 06.12.2018 16:11