Sequences from confirmation amplicon


Confirmation sequences for line 817E04 and hit At1g29670 are shown.
Line ID 817E04
Locus At1g29670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10377196
Primers used for PCR HM09 - 8474, show primer details
   
Sequence Name 43-K029126-022-817-E04C-8409
GenBank Accession LN504723 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K029126-022-817-E04C-8409
CGGTTTAAACTACTGTTTAAACTATCATTGCTGATCTGGTTTGCTCCAATTCCACTCAAT
GCAAACTTTCGAGCCCCATAGTTGTATAATGCCTGCACAAACCCCGAAAAATGGAAGTAA
AACTCCGATGAAAATTTTACAAAATTGCATAAATTTTATTATTCATCCTTTATATTATTT
TCCAAAAATAAAAACATTTCTCACAAGATAGGTCACTCAGAAAAAGCTTAAGCTAGAAAC
TTTATATTGTGTACTACAAGTGAAGACCAAAATTGGGTTTAAATTAACTGGTGTGAGACC
TTAACTTGTAGTCTTGTACTATAAAGCTATAAATTGCTATTTTTGTAATTCTCTGCCTCA
AAAAGTCGTATTTTCATATTAGAAACCAAATCGTATTTAAATGAAAAGAGTCATAATACG
AAAGTTAAAAGATAGACCAAACCCACGTCTAAAAACCTTACTATCACTGCTAAATGGGT
   
Sequence Name 44-K029126-022-817-E04C-HM09
GenBank Accession LN505065 [GenBank]
Sequencing primer gene specific primer, HM09: 5'-TCTCGAGTTTACCCATTTAGCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K029126-022-817-E04C-HM09
ATAGTAAGGTTTTTAGACGTGGGTTTGGTCTATCTTTTAACTTTCGTATTATGACTCTTT
TCATTTAAATACGATTTGGTTTCTAATATGAAAATACGACTTTTTGAGGCAGAGAATTAC
AAAAATAGCAATTTATAGCTTTATAGTACAAGACTACAAGTTAAGGTCTCACACCAGTTA
ATTTAAACCCAATTTTGGTCTTCACTTGGAGTACACAATATAAAGTTTCTAGCTTAAGCT
TTTTCTGAGGGGACCTATCTGGTGAGAAATGTTTTTATTTTTGGAAAATAATATAAGGGA
TGAATAATAAAATTTATGCAATTTTGTAAAATTTTCATCGGAGTTTTACTTCCATTTTTC
GGGGTTTGTGCAGGCATTATACAACTATGGGGCTCGAAAGTTTGCATTGAGTGGAATTGG
AGCAAACCAGATCAGCAATGATAGTTTAAACAGTAGTTTAAACCGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCT

Last Updated on 06.12.2018 16:11