Sequences from confirmation amplicon


Confirmation sequences for line 818H06 and hit At5g43810 are shown.
Line ID 818H06
Locus At5g43810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17614548
Primers used for PCR IF15 - 8474, show primer details
   
Sequence Name 40-K032738-022-818-H06C-8409
GenBank Accession LN503612 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K032738-022-818-H06C-8409
CCATTTACAATTGACTTCGGTTCAAACAATGCAACAAGAAAAGAAAAAAAGATGAATATT
ATTACCATGCCTGAGACTTCACACATCTGACCAAGTTCATTACAAAATCCACGAGCAACG
TTTTCTTGAACGCTGCGTGAGAAGTTAACACAGGCCCATCTGCTCACAGTCATCCCGTTG
ATCATTTTCTTGTTCATCATATTCCACTGACCAACTTGCGGGAGACAATCTTTTTCTTTC
CCGTTCTCGTGATACTTAAGCTGTAGCAAGCAAAGAGAGACGAATTAACATAACAGTATT
CTCATAGTATGAGCATTCAGAGATCTGTTTAGGTTTTTTACCCATGGAGCTGGAAGAATA
CGAGCTTCAACAGAAGCTAACTTTTCGCTTATGTTCATGCCAAACTCCTTTGCATATGGA
TCTTGATCATATGCGTTGTGTTGGACAGTCTATAAAACATTTTTGACAACAAGATTAATC
CTCACTTCTAAGTTCTAACTCACAAAGAACCATATAAGATACATGACTAAAAAAATACCC
GCAAAATATCGTTTTCTCTGTCCCTCGGCCTTTGGCATGTAACTTTCAAGAGAGCAGTAA
TCTGCTTCTCATTCAACCTTTTCGTGTACCGTTGTCCCTCGACAATTTTGCATGCCTACA
TTGGAAATTGAATCAGCGAAAATAGCCAAGAGCTGAATGTGA
   
Sequence Name 72-K032738-022-818-H06C-IF15
GenBank Accession LN512657 [GenBank]
Sequencing primer gene specific primer, IF15: 5'-TGTTTCAAGACTTCACATTTCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K032738-022-818-H06C-IF15
TCATTTCCATGTAGGCATGCAAAATTGTCGAGGGACAACGGTACACGAAAAGGTTGAATG
AGAAGCAGATTACTGCTCTCTTGAAAGTTACATGCCAAAGGCCGAGGGACAGAGAAAACG
ATATTTTGCGGGTATTTTTTTAGTCATGTATCTTATATGGTTCTTTGTGAGTTAGAACTT
AGAAGTGAGGATTAATCTTGTTGTCAAAAATGTTTTATAGACTGTCCAACACAACGCATA
TGATCAAGATCCATATGCAAAGGAGTTTGGCATGAACATAAGCGAAAAGTTAGCTTCTGT
TGAAGCTCGTATTCTTCCAGCTCCATGGGTAAAAAACCTAAACAGATCTCTGAATGCTCA
TACTATGAGAATACTGTTATGTTAATTCGTCTCTCTTTGCTTGCTACAGCTTAAGTATCA
CGAGAACGGGAAAGAAAAAGATTGTCTCCCGCAAGTTGGTCAGTGGAATATGATGAACAA
GAAAATGATCAACGGGATGACTGTGAGCAGATGGGCCTGTGTTAACTTCTCACGCAGCGT
TCAAGAAAACGTTGCTCGTGGATTTTGTAATGAACTTGGTCAGATGTGTGAAGTCTCAGG
CATGGTAATAATATTCATCTTTTTTTCTTTTCTTGTTGCATTGTTTGAACCGAAGTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAAACAATTATTCTAATTC
Primers used for PCR FQ20 - 3144, show primer details
   
Sequence Name 01-K033039-022-818-H06C-FQ20
GenBank Accession LN486463 [GenBank]
Sequencing primer gene specific primer, FQ20: 5'-GGGATACATTTGCCAGATACTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K033039-022-818-H06C-FQ20
TTTGTGAGAACATTGAGATATCAAACCAAGCTCGGTTTCACAGATTCTCTTAAGATCACC
TGAGATTACAAGAAACATTTCGTTTTCATCAAAACCATTACTAAAGAAGGCAAAAGGGTT
AAGTTTATGGATGTCGAAATAATACCATAAAGTGAACCGTTGTTATCAGGTAATATTGCC
AGCAGAAGCTCTAACTCTTTGCCTTTGGTTTTGTTCATTGAAGTGTGATAAACATGCTTT
AGAGCTTTCTCGACTTGATCGGGCCTCGCACTATATATTGGTATCACGGGTTCTGGATTA
AACTCCTACAATGATAAACGAAGCAAAGCTTTCTGAATGATAAACACTGATAGTTTAAAC
CGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAAC
TTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGT
CAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTT
CCACGATGCTCCTCGTGGGTGGG
   
Sequence Name 33-K033039-022-818-H06C-3144
GenBank Accession LN500893 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K033039-022-818-H06C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAACTATCAGTGTTTATCATTCAGAAA
GCTTTGCTTCGTTTATCATTGTAGGAGTTTAATCCAGAACCCGTGATACCAATATATAGT
GCGAGGCCCGATCAAGTCGAGAAAGCTCTAAAGCATGTTTATCACACTTCAATGAACAAA
ACCAAAGGCAAAGAGTTAGAGCTTCTGCTGGCAATATTACCTGATAACAACGGTTCACTT
TATGGTATTATTTCGACATCCATAAACTTAACCCTTTTGCCTTCTTTAGTAATGGTTTTG
ATGAAAACGAAATGTTTCTTGTAATCTCAGGTGATCTTAAGAGAATCTGTGAAACCGAGC
TTGGTTTGATATCTCAATGTTGTCTCACAAAACATGTGTTCAAGATTAGCAAACAGTATC
TGGCAATGGTATCC

Last Updated on 06.12.2018 16:11