Sequences from confirmation amplicon


Confirmation sequences for line 819D01 and hit At5g51030 are shown.
Line ID 819D01
Locus At5g51030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20749107
Primers used for PCR 17xq - 8474, show primer details
   
Sequence Name 79-K119830-0022-819-D01C-17xq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 17xq: 5'-AAAACTTTGGTTCGTATTGAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K119830-0022-819-D01C-17xq
TTCCATAACAGATGCTATAACTAAAAATAGGAATCAGATGAATTTGAACTGAGAGGCCAC
AAAAAAATTACGCAGAAAGTGGGGGATTGCACCTTTTGACCCACAAAAAGGATGAGAAAA
TTATGTGAGAGGGAGATGAGAACGACGACGAAGCTCTGTGTTCTTTCTTGCCTCCTCTTA
TCGTGAAAATAAACATACTTAGGATCGTAGAAGAGAGAAAATAAGAAGAAAGATGGTAGG
AAGCAAAGAAAAATCGAAAGAGAAACGAGATAAGAGACTCCAAGAGATCTCCCTCCTTCG
TACTATTCCCTATTCTGATCACCAGAGGTATCAATATTCCCATTCTAAATTTTTATGTCT
TCATATCTATGAAATTTTGATTATAATAAATGTTTGTATATATCCCTTACTCAGTAGGAA
GAATTACATTTAGGTCTAGTCAAAACATATAAACTAGTTTACTTGGTACAAGCTTATCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
AAGATATCCGCAGCGTTATT
   
Sequence Name 55-K119829-0022-819-D01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K119829-0022-819-D01C-8409
TTGAATATATCCTGATAAGCTTGTACCAAGTAAACTAGTTTCTATGTTTTGACTAGACCT
AAATGTAATTCTTCCTACTGAGTAAGGGATATATACAAACATTTATTATAATCAAAATTT
CATAGATATGAAGACATAAAAATTTAGAATGGGAATATTGATACCTCTGGTGATCAGAAT
AGGGAATAGTACGAAGGAGGGAGATCTCTTGGAGTCTCTTATCTCGTTTCTCTTTCGATT
TTTCTTTGCTTCCTACCATCTTTCTTCTTATTTTCTCTCTTCTACGATCCTAAGTATGTT
TATTTTCACGATAAGAGGAGGCAAGAAAGAACACAGAGCTTCGTCGTCGTTCTCATCTCC
CTCTCACATAATTTTCTCATCCTTTTTGTGGGTCAAAAGGTGCAATCCCCCACTTTCTGC
GTAATTTTTTTGTGGCCTCTCATTTCAAATTCATCTGATTCCTATTTTTAGTTATAGCAT
CTGTTATGGGAACATAAATTAGCTGGGGCCTAGCTAGGGTGTGGGCTCTCAATACGAACC
AAAGTTT

Last Updated on 06.12.2018 16:11