Sequences from confirmation amplicon


Confirmation sequences for line 819H03 and hit At4g12370 are shown.
Line ID 819H03
Locus At4g12370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7332220
Primers used for PCR MA71 - 8474, show primer details
   
Sequence Name 19-K102512-0022-819-H03C-8409
GenBank Accession LN494710 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K102512-0022-819-H03C-8409
ATTTATCATATGATGCGTTTACTTGCGTGTTGCTAGCATTTAAGCATGTTTCGTATCGTA
TCACCACTACGAGCACTTGCCATCCCAAAGCAACCGAGTGGGTTACTGAGGATCTACAAT
TCCATCGTCGCTTCCGCAGTGAAACACTTAACCACAGCAATGTTGTCTATGCCAAACGTC
GCTTCTATTGCCTTGACGGTCAAGGAAGCTTATATTACTTTGATCCGTCTTCTCGAAGAT
GGGATTTTAGTTACACCTATTTACTGCCATGTCCTTATATCTCGGATAGATTTAGTTACC
AGTATGAGCGGAAGAAGAAGAGAATTTTCTTGGCTGTGCGGAAAGGAGTGTTCTTTAAGA
TATTTACATGTGATGGTGAGAAGCCGATAGTGCATAAGTTAGAAGATATCAATTGGGAGG
AGATCAATAGTACTACGATTGATGGATTGACAATCTTTACGGGTCTTTATTCCTCTGAGG
TGAGACTTAATCTACCATGGATGAGGAATAGTGTTTACTTTCCTAGACTTCGTTTTATGN
TCAAGCG
   
Sequence Name 38-K102513-0022-819-H03C-MA71
GenBank Accession LN502933 [GenBank]
Sequencing primer gene specific primer, MA71: 5'-ACGCTTGACATTAAAACGAAGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K102513-0022-819-H03C-MA71
TAGATTAGTCTCACCTCAGAGGAAAAAGACCCGTAAAGATTGTCAATCCATCAATCGTAG
TACTATTGATCTCCTCCCAATTGATATCTTCTAACTTATGCACTATCGGCTTCTCACCAT
CACATGTAAATATCTTAAAGAACACTCCTTTCCGCACAGCCAAGAAAATTCTCTTCTTCT
TCCGCTCATACTGGTAACTAAATCTATCCGAGATATAAGGACATGGCAGTAAATAGGTGT
AACTAAAATCCCATCTTCGAGAAGACGGATCAAAGTAATATAAGCTTCCTTGACCGTCAA
GGCAATAGAAGCGACGTTTGGCATAGACAACATTGCTGTGGTTAAGTGTTTCACTGCGGA
AGCGACGATGGAATTGTAGATCCTCAGTAACCCACTCGGTTGCTTTGGGATGGCAAGTGC
TCGTAGTGGTGATACGATACGAAACATGCTTAAATGCTAGCAACACGCAAGTAAACGCAT
CATATGATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTC

Last Updated on 06.12.2018 16:11