Sequences from confirmation amplicon


Confirmation sequences for line 820B09 and hit At3g51690 are shown.
Line ID 820B09
Locus At3g51690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19177298
Primers used for PCR GF16 - 8474, show primer details
   
Sequence Name 12-K035349-022-820-B09C-GF16
GenBank Accession LN491481 [GenBank]
Sequencing primer gene specific primer, GF16: 5'-ATGTCGACGACTAAATGTTTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K035349-022-820-B09C-GF16
CATTCTTGGCTTCTTCCTGAGGGTTCCCATCTTTGTCTGTTATAAGAACTTTCAAACCAG
CTCTTGACTTCACCTTGGATATAGCAACAAACATCTGTCGTCCATGAGAGAACACTTGTC
TTGGTAGATATAGACCAACTTTGGAAAGTGTATGCACTTGACTTTCATCTATAGTCATCG
CGAACGCAAGTTTCAAAGGATATTGCGTACGCCGCATTTGAAGAGGAAAATTTGTTTCTG
TGGGGTATGAAGCTATCCTAGGGATCCAGATTTTCTTACCATGCTTATTACCTGCTATAA
TCATAGCCTCAAGCAAAAACCCACACAGCCGAGTAATTTGAAGTCTTGTCCCCGTAAAAA
AACCACGGCTAGGATCAAGGTCCCTAAGTAACATGACAGGAGCACCAACCTTTAACCTCA
GCTTAAAATCAGGCAATCCGGGGACTTTGATGCTATTCAAAACCTCTAGAGGGACAAACA
TGTCGTCATTTGGGCTTGGAGAAATACTGTCAGTGCTGAGACATTCTTTCTCTTCATTAT
TAGTCGATAATTTGGATCAATTCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 04-K035349-022-820-B09C-8409
GenBank Accession LN487820 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K035349-022-820-B09C-8409
CCATTTAAATTGAATATATCCTGAAGAATTGATCCAAATTATCGACTAATAATGAAGAGA
AAGAATGTCTCAGCACTGACAGTATTTCTCCAAGCCCAAATGACGACATGTTTGTCCCTC
TAGAGGTTTTGAATAGCATCAAAGTCCCCGGATTGCCTGATTTTAAGCTGAGGTTAAAGG
TTGGTGCTCCTGTCATGTTACTTAGGGACCTTGATCCTAGCCGTGGTTTTTTTACGGGGA
CAAGACTTCAAATTACTCGGCTGTGTGGGTTTTTGCTTGAGGCTATGATTATAGCAGGTA
ATAAGCATGGTAAGAAAATCTGGATCCCTAGGATAGCTTCATACCCCACAGAAACAAATT
TTCCTCTTCAAATGCGGCGTACGCAATATCCTTTGAAACTTGCGTTCGCGATGACTATAG
ATGAAAGTCAAGTGCATACACTTTCCAAAGTTGGTCTATATCTACCAAGACAAGTGTTCT
CTCATGGACGACAGATGTTTGTTGCTATATCCAAGGTGAAGTCAAGAGCTGGTTTGAAAG
TTCTTATAACAGACAAAGATGGGAACCCTCAGGAAGAAGCCAAGAATGTTGTGTTCAAGG
AGTTGTTTCAAAACATTTAGTCGTCGAC

Last Updated on 06.12.2018 16:11