Sequences from confirmation amplicon


Confirmation sequences for line 822A07 and hit At3g10910 are shown.
Line ID 822A07
Locus At3g10910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3412974
Primers used for PCR 20gm - 8474, show primer details
   
Sequence Name 27-K112586-0022-822-A07C-20gm
GenBank Accession LN498460 [GenBank]
Sequencing primer gene specific primer, 20gm: 5'-CTGCTTTACTCTGCGCTTTAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K112586-0022-822-A07C-20gm
GCATACTCGGAGATTCACTTCGGATGATCAAGTCTCAAACGCTTCAAACGCAAACGCAAA
TTTGGGACGTTTAGCGGCTGCCACGGGTCTCAAGAAACAGGCGTTGAAACAAATCCCCGT
GGGACTGTACGGATCAGGAATCATAGACATGAAAGCTACGGAGTGTTTGATCTGTCTGGG
AGATTTCGAAGATGGAGAAAAAGTTAGGGTTTTACCTAAATGTAACCATGGCTTCCACGT
GAGGTGCATCGATACTTGGTTGCTCTCACGTTCCTCTTGCCCCACTTGTAGACAATCGCT
ACTCCTTGAGCAGCCTTCGCCGATGGCTGTTTCCCGGCGGGACGAGGACATGGTAGTTTC
CATCGTATAGGGACATGATCGGTGATTTCTTAATTATGCTCTCTCAAGTGGGCTTGGTTT
TTGTTGGTTGGAATTGTTTGTGTGTGTGCAAACTAATGTGATTTATTTTGACAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATA
TCCGCA
   
Sequence Name 01-K112585-0022-822-A07C-8409
GenBank Accession LN486642 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K112585-0022-822-A07C-8409
CATTTCATTGAATATATCCTGTCAAATAAATCACATTAGTTTGCACACACACAAACAATT
CCAACCAACAAAAACCAAGCCCACTTGAGAGAGCATAATTAAGAAATCACCGATCATGTC
CCTATACGATGGAAACTACCATGTCCTCGTCCCGCCGGGAAACAGCCATCGGCGAAGGCT
GCTCAAGGAGTAGCGATTGTCTACAAGTGGGGCAAGAGGAACGTGAGAGCAACCAAGTAT
CGATGCACCTCACGTGGAAGCCATGGTTACATTTAGGTAAAACCCTAACTTTTTCTCCAT
CTTCGAAATCTCCCAGACAGATCAAACACTCCGTAGCTTTCATGTCTATGATTCCTGATC
CGTACAGTCCCACGGGGATTTGTTTCAACGCCTGTTTCTTGAGACCCGTGGCAGCCGCTA
AACGTCCCAAATTTGCGTTTGCGTTTGAAGCGTTTGAGACTTGATCATCCGAAGTGAATC
TCCGAGTTATGCGTAGCACACATCGCANGGCAGAGTTGAGGCTTAGAGCACAGATNAAAG
CGCAAGNTAAAGCAG

Last Updated on 06.12.2018 16:11