Sequences from confirmation amplicon


Confirmation sequences for line 822B01 and hit At4g13050 are shown.
Line ID 822B01
Locus At4g13050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7618530
Primers used for PCR BF58 - 8474, show primer details
   
Sequence Name 73-K032009-022-822-B01C-8409
GenBank Accession LN512826 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K032009-022-822-B01C-8409
TTACAATTGAATAATTCAGTGTTCTTCGGTTCCAAGATGAAAAAGTCAAAACCTTGTAGC
ACGTCCAATAACTTCACCAGTAGCACAATCCTTTAAAATCCAATCACGTCTAGTTCCAAT
CCTTCCTTCACTCTGACACCAAGTCTCAATCTCAACCACATCACTCCTATAACCAAAACA
AGAAAGCTTAAAACTTTCAACTCCAAACCAGAAAATCCTCAAACCACTCAAAGCCTTACC
AAGCTGGATACTTGTAAATCTCAATGTGCATTCTTGCAGTAACCCAAATAAGATGCAATT
TCCTCATGGTAAGTGTTGTAGCAAATCCATCAGTAGAAAATCCAACATTCTGAACATGAT
TACATGCCACTTCCTACACAAATCCATACTCAAAACATCAATCACACAAAGACATAACCA
ACTAAACAAACATTGCAACACACAAGAATAGTGTTTAGTATATTGAGTACACACAAGACA
CAAAATCCCCAAACCTGCAAGAGATAGNCAATTG
   
Sequence Name 74-K032009-022-822-B01C-BF58
GenBank Accession LN512998 [GenBank]
Sequencing primer gene specific primer, BF58: 5'-ACAATTGCTAATCTCTTGCAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K032009-022-822-B01C-BF58
CATATACTAAACACTATTCTTGTGTGTTGCAATGTTTGTTTAGTTGGTTATGTCTTTGTG
TGATTGATGTTTTGAGTATGGATTTGTGTAGGAAGTGGCATGTAATCATGTTCAGAATGT
TGGATTTTCTACTGATGGATTTGCTACAACACTTACCATGAGGAAATTGCATCTTATTTG
GGTTACTGCAAGAATGCACATTGAGATTTACAAGTATCCAGCTTGGTAAGGCTTTGAGTG
GTTTGAGGATTTTCTGGTTTGGAGTTGAAAGTTTTAAGCTTTCTTGTTTTGGTTATAGGA
GTGATGTGGTTGAGATTGAGACTTGGTGTCAGAGTGAAGGAAGGATTGGAACTAGACGTG
ATTGGATTTTAAAGGATTGTGCTACTGGTGAAGTTATTGGACGTGCTACAAGGTTTTGAC
TTTTTCATCTTGTGAACCGAAGAACACTGAATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11