Sequences from confirmation amplicon


Confirmation sequences for line 822F12 and hit At1g79030 are shown.
Line ID 822F12
Locus At1g79030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29732698
Primers used for PCR 42op - 8474, show primer details
   
Sequence Name 17-K114972-0022-822-F12C-42op
GenBank Accession LN493970 [GenBank]
Sequencing primer gene specific primer, 42op: 5'-ATTAACAACAGTAGAAGGCGCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114972-0022-822-F12C-42op
CTCGGTTTCATCTTCGACAGGAACTGAAGGATACTCAAATTCCCTTGAATAGTCTTCTTC
TATGATTGTTTCTTTCGGTTTCTTTGGTTCCTCGTGTTGTGGTTTTTCACTTAGATTGTC
ACACCATTGAAGAAGACAATTTAATATATCGTTGGACAAGAAAGCAAGATTTATTGAGAG
GAAAAGACCAAGCCATCCAAGTCTGACTTTGACACAATACATCGCATATAAGCCCGCCAC
AAGAACCACCACGCGAGCATGATTTAAGGAGAATAAATAGTCGAGACCATGCATATGGCT
TCGGAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 08-K114971-0022-822-F12C-8409
GenBank Accession LN489848 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114971-0022-822-F12C-8409
ATGCATGGTCTCGACTATTATTCTCCTTAATCATGCTCGCGTGGTGGTTCTTGTGGCGGG
CTTATATGCGATGTATTGTGTCAAAGTCAGACTTGGATGGCTTGGTCTTTTCCTCTCAAT
AAATCTTGCTTTCTTGTCCAACGATATATTAAATTGTCTTCTTCAATGGTGTGACAATCT
AAGTGAAAAACCACAACACGAGGAACCAAAGAAACCGAAAGAAACAATCATAGAAGAAGA
CTATTCAAGGGAATTTGAGTATCCTTCAGTTCCTGTCGAAGATGAAACCGAGACAAAGAT
TCACGAGAATAAGTCTTCTGCTAAACCAACTGCGCCTTCTATGATTGTTAA

Last Updated on 06.12.2018 16:11