Sequences from confirmation amplicon


Confirmation sequences for line 823D08 and hit At4g26740 are shown.
Line ID 823D08
Locus At4g26740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13474078
Primers used for PCR DB31 - 8474, show primer details
   
Sequence Name 38-K030142-022-823-D08C-8409
GenBank Accession LN502870 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K030142-022-823-D08C-8409
GCATTTACAATTGAATATATCCTGTCTGGCATAGCCATTTACAATCACAGCATTAGCCTT
CCTTTTTCTTACCCTTTCATTAGTTTATTGAATGCATGTGTTAAACTAAAGTATTAGTCA
ATGTTGTTGTAGTTATAATGTTTGGATCTACATGTATGTATTAGGATCGCAGGCAAAATA
GAGTGGGGACTGTTGTACTTGCTAGCAAGGGATGAAGAAGGGTTTTTGTCAAAAGAAGCT
ATTAGGCGGTGTTTCGATGGAAGCTTGTTCGAGTACTGTGCCAAAATCTACGCTGGTATC
AGTGAAGACAAGACAGCATACTACTAAAAGTATCCTTTATGTTAAGTAATTGATCGAGCC
ATTTTAAGCTAAATAAT
   
Sequence Name 38-K030142-022-823-D08C-DB31
GenBank Accession LN502871 [GenBank]
Sequencing primer gene specific primer, DB31: 5'-CGATTATTAGCTTAAAATGGCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K030142-022-823-D08C-DB31
ATGCTGTCTTGTCTTCACTGATACCAGCGTAGATTTTGGCACAGTACTCGAACAAGCTTC
CATCGAAACACCGCCTAATAGCTTCTTTTGACAAAAACCCTTCTTCATCCCTTGCTAGCA
AGTACAACAGTCCCCACTCTATTTTGCCTGCGATCCTAATACATACATGTAGATCCAAAC
ATTATAACTACAACAACATTGACTAATACTTTAGTTTAACACATGCATTCAATAAACTAA
TGAAAGGGTAAGAAAAAGGAAGGCTAATGCTGTGATTGTAAATGGCTATGCCAGACAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATT

Last Updated on 06.12.2018 16:11