Sequences from confirmation amplicon


Confirmation sequences for line 823G03 and hit At3g10780 are shown.
Line ID 823G03
Locus At3g10780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3376212
Primers used for PCR 21os - 8474, show primer details
   
Sequence Name 65-K114954-0022-823-G03C-21os
GenBank Accession LN511458 [GenBank]
Sequencing primer gene specific primer, 21os: 5'-GACCAGTCACATCACTCAGTCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K114954-0022-823-G03C-21os
GGGTATCAGAGCCAAGACTGGGATTCTGTTGCTAAAAAGAAAAGATCGAGGCAAGTCCTT
CATTTCCAATTCTAATATATGATTATAATAATCTAAAAAAACACATGTAATTGAATATAT
GGTTCCAGGGTGTGGAGCTTGAGATTCGAAGATCTACAGAATACGCAAGTGCTATTAGGG
CCAACATACTCTATTTGAGAATCAGGTTATTAATAACTTTCATATCAATCTTACGTATAT
ATGTGGAATACGCAAAAAAGGATTTAATTAAGATTCTCTTATAAAAAAATGGTGCAGGGA
AGCATATATGAGGGAAATAAACGAGAAGACAAACACGAGGGTCAATCAGCAATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCACANTTATA
   
Sequence Name 31-K114953-0022-823-G03C-8409
GenBank Accession LN500281 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114953-0022-823-G03C-8409
GTTATTTCCCTCTATATGCTTCCCTGCACCATTTTTTTATAAGAGAATCTTAATTAAATC
CTTTTTTGCGTATTCCACATATATACGTAAGATTGATATGAAAGTTATTAATAACCTGAT
TCTCAAATAGAGTATGTTGGCCCTAATAGCACTTGCGTATTCTGTAGATCTTCGAATCTC
AAGCTCCACACCCTGGAACCATATATTCAATTACATGTGTTTTTTTAGATTATTATAATC
ATATATTAGAATTGGAAATGAAGGACTTGCCTCGATCTTTTCTTTTTTAGCAACAGAATC
CCAGTCTTTGGCTCTGATACCCATCTTCCAATCAAGACTGACGATNACAGAGCTATTGAC
TGAGTGATGGGACTGGT

Last Updated on 06.12.2018 16:11