Sequences from confirmation amplicon


Confirmation sequences for line 823G12 and hit At1g52460 are shown.
Line ID 823G12
Locus At1g52460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19549566
Primers used for PCR 31tq - 8474, show primer details
   
Sequence Name 26-K116964-0022-823-G12C-31tq
GenBank Accession LN498097 [GenBank]
Sequencing primer gene specific primer, 31tq: 5'-AAAAATAATATTCCGTTCATGTGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116964-0022-823-G12C-31tq
TGTAGATATGTANAATACAAAACAAATCATGTGATATAAACGTTACTCAGAGAAATGCTC
ATCCTTATCATGCAGCCATACTATAGTGACTCTATGCTCTCCTTTAGGTTGCACAACAAA
ATCTGCTCTGTTGCTACTACCTGCAAAGATTTTACCATAAAACAACGTCATTAACCAAGC
AAAATTGTCTGAGAGTAGTAGCCCACAACTGAATAAAAACTACATAAAAGAGAAGAAAAA
TATCATCGGAGATTTTTAATATTCATTCGAAAATTAATATCAGCAAATGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 08-K116963-0022-823-G12C-8409
GenBank Accession LN489900 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K116963-0022-823-G12C-8409
TTTGCTGATATAATTTTCGAATGAATATTAAAAATCTCCGATGATATTTTTCTTCTCTTT
TATGTAGTTTTTATTCAGTTGTGGGCTACTACTCTCAGACAATTTTGCTTGGTTAATGAC
GTTGTTTTATGGTAAAATCTTTGCAGGTAGTAGCAACAGAGCAGATTTTGTTGTGCAACC
TAAAGGAGAGCATAGAGTCACTATAGTATGGCTGCATGATAAGGATGAGCATTTCTCTGA
GTAACGTTTATATCACATGATTTGTTTTGTATTATTACATATCTTACATTTCTTCTAATC
CAAAAGAAAAAAAATTCATCTCACATGAACGGATAATTTATTTTT

Last Updated on 06.12.2018 16:11