Sequences from confirmation amplicon


Confirmation sequences for line 823H04 and hit At5g41910 are shown.
Line ID 823H04
Locus At5g41910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16778519
Primers used for PCR 07ty - 8474, show primer details
   
Sequence Name 33-K117118-0022-823-H04C-07ty
GenBank Accession LN501157 [GenBank]
Sequencing primer gene specific primer, 07ty: 5'-GTCTCTTCTTTCACACCTGCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117118-0022-823-H04C-07ty
CTTCTCTATGATCCATTGTTTTGAGTATTGTGTGATTGAAATTTTGTCTCTATCTGCAGC
AATTCTCTTGTGATGGAGCTTGATAATATGGCTAAGTTGTCTGATAAATGCAACATTCAA
GTCCCCATTGAGGTTCTTAAGTGAGAACAAGTCCCTTTTTAGACTCTTTAGAGAATTTTT
GTTGCTCTCTTATGTTTATATATCTTTGTTTGTTGTAGCTTGATTGATGATGGGAAGAAC
CCAGATGAGTTTACAAAAGATGTTCTTAATAAGAACTGCATTGCTAAAACAATTGATGTT
CATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG
   
Sequence Name 11-K117117-0022-823-H04C-8409
GenBank Accession LN491295 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117117-0022-823-H04C-8409
GCATTTCATTGAATATATCCTGATGAACTCAATTGTTTTAGCAATGCAGTTCTTATTAAG
AACATNCTTTTGTAAACTCATNCTGGGTTCTTCCCATCATCAATCAAGCTACAACAAACA
AAGATATATAAACATAAGAGAGCAACAAAAATTCTCTAAAGAGTCTAAAAAGGGACTTGT
TCTCACTTAAGAACCTCAATGGGGACTTGAATGTTGCATTTATCAGACAACTTAGCCATA
TTATCAAGCTCCATCACAAGAGAATTGCTGCAGATAGAGACAAAATTTCAATCACACAAT
ACTCAAAACAATGGATCAATAGAGAAGAAGAAAAGTTACAGGCGTTGGAGGAGGTGAAGC
TGAGAAGCAGGTGTGAAGGAAAGAGA

Last Updated on 06.12.2018 16:11