Sequences from confirmation amplicon


Confirmation sequences for line 824B07 and hit At5g05520 are shown.
Line ID 824B07
Locus At5g05520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1635058
Primers used for PCR 26wm - 8474, show primer details
   
Sequence Name 47-K118924-0022-824-B07C-26wm
GenBank Accession LN506350 [GenBank]
Sequencing primer gene specific primer, 26wm: 5'-CTGCATTATGGGGATTCAAGACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118924-0022-824-B07C-26wm
GACGATGAGAGTGGTGACACATATGAACGGGATTTTGTGGGAGGAGATGTTGCGGTTACT
GCATTTGCAGACCTTTCTTTTGATTTCCCATTGAAATGGTTCAGAGACAGAGGAATCCAC
GGACATGTGTTTGCATGTGCCGGGAATATGGCGGAATTATCAGAGAACAAGTATAGGAAC
TTCACAGCTCCAAAGTTGTTGGAGACATTCAGAAGCTCAGTTGGTGCAGGAATCGTGGTA
CCAACTAGTCTATTTCGCATGGAGGTTAGTTAGTTCTCTTGTTTCTCTTTTCTTATCAAG
GATCATAAAAAAATTGCAATCCTTTTAACAGATATTGATTGTTCTTCTTTTGTTATGTGA
TCTCTTATGCCAGCTCAACTACTGCCACATACTGAAGAAACAAGAACACGATCGAGCAAA
ATCTGGATTTTTCATGACATTTTCAACATCATCTTAATGCTACTTTTGATGATATCTTAA
TTTTGGCTTTGCACTCACAGTGTCATGGCGGAGTCTTTTGTTTTGTTTCCAGGAGCTTTC
ATTGTTTAGGTTGTTGTTTGGAATTGAAAACTGTGCAAATTTTAGAGAGAGAGATTACCT
GATGTGCGGATTGTTCTTGGGAAGGAGATAGATCAAGAAATAAAAATTAACTAAAGTTGA
TTTGTTTTGAATTAAATTTTAAATTAGTAATAAACTTTTTGTTTTCCTTCCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTT
   
Sequence Name 23-K118923-0022-824-B07C-8409
GenBank Accession LN496774 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118923-0022-824-B07C-8409
CATTTACAATTGGAGGAAAAAAAAAGTTTATTACTAATTTAAAATTTAATTCAAAACAAA
TCAACTTTAGTTAATTTTTATTTCTTGATCTATCTCCTTCCCAAGAACAATCCGCACATC
AGGTAATCTCTCTCTCTAAAATTTGCACAGTTTTCAATTCCAAACAACAACCTAAACAAT
GAAAGCTCCTGGAAACAAAACAAAAGACTCCGCCATGACACTGTGAGTGCAAAGCCAAAA
TTAAGATATCATCAAAAGTAGCATTAAGATGATGTTGAAAATGTCATGAAAAATCCAGAT
TTTGCTCGATCGTGTTCTTGTTTCTTCAGTATGTGGCAGTAGTTGAGCTGGCATAAGAGA
TCACATAACAAAAGAAGAACAATCAATATCTGTTAAAAGGATTGCAATTTTTTTATGATC
CTTGATAAGAAAAGAGAAACAAGAGAACTAACTAACCTCCATGCGAAATAGACTAGTTGG
TACCACGATTCCTGCACCAACTGAGCTTCTGAATGTCTCCAACAACTTTGGAGCTGTGAA
GTTCCTATACTTGTTCTCTGATAATTCCGCCATATTCCCGGCACATGCAAACACATGTCC
GTGGATTCCTCTGTCTCTGAACCATTTCAATGGGAAATCAAAAGAAAGGTCTGCAAATGC
AGTAACCGCAACATCTCCTCCCACAAAATCCCGTTCATATGTGTCACCACTCTCATCGTC
CTGTACTTCCCTCCTTGGTTCGTTAGGACCCAATCCCCTAGTCTTGAATCC

Last Updated on 06.12.2018 16:11