Sequences from confirmation amplicon


Confirmation sequences for line 824E02 and hit At3g57530 are shown.
Line ID 824E02
Locus At3g57530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21298993
Primers used for PCR TR07 - 8474, show primer details
   
Sequence Name 12-K027365-022-824-E02C-8409
GenBank Accession LN491421 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K027365-022-824-E02C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTCCAGTCTTTACGGCGAGTTCTGTATGTTG
ACTCATTGCTGTTAGCATCCTAATGTTGTTACTTTGAAGGAGACTTATGAGGATGAGCAT
GCTGTTCATTTGGTTATGGAGCTTTGTGAAGGTGGTGAATTGTTTGATAGGATTGTTGCT
AGAGGGCATTATACTGAGAGAGCTGCTGCTGCTGTCACTAAGACCATCATGGAAGTTGTT
CAGGTTCGTCAAGGTTTTTTTGCTGTTTATTGTATCTCTTTAGTCTTTACTTGTTGCAGA
ATTGCAATGGCCTCGTATAATGTCTTGGTTTGTTACTGGTTTCAGTTTCTTGGACATTAT
TGACTTGTAGCATTG
   
Sequence Name 16-K027365-022-824-E02C-TR07
GenBank Accession LN493289 [GenBank]
Sequencing primer gene specific primer, TR07: 5'-CCCTTTAAAGCAATGCTACAAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K027365-022-824-E02C-TR07
TAATGTCCAAGAAACTGAAACCAGTAACAAACCAAGACATTATACGAGGCCATTGCAATT
CTGCAACAAGTAAAGACTAAAGAGATACAATAAACAGCAAAAAAACCTTGACGAACCTGA
ACAACTTCCATGATGGTCTTAGTGACAGCAGCAGCAGCTCTCTCAGTATAATGCCCTCTA
GCAACAATCCTATCAAACAATTCACCACCTTCACAAAGCTCCATAACCAAATGAACAGCA
TGCTCATCCTCATAAGTCTCCTTCAAAGTAACAACATTAGGATGCTAACAGCAATGAGTC
AACATACAGAACTCGCCGTAAAGACTGGAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11