Sequences from confirmation amplicon


Confirmation sequences for line 824F01 and hit At1g13910 are shown.
Line ID 824F01
Locus At1g13910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4756542
Primers used for PCR 62by - 8474, show primer details
   
Sequence Name 79-K108523-0022-824-F01C-62by
GenBank Accession LN513837 [GenBank]
Sequencing primer gene specific primer, 62by: 5'-TGAATGTCAAAGATTACAAGTGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K108523-0022-824-F01C-62by
GATTCGCCCGNAAAATGATTCTCCTGAATATGTAAGTACTGGAGCCCTTTAAGATTTGCA
AGTTCTTTGGGGATTTCTCCTTTGAAATCGTTAAAACTCAGGTACCTGCAATATTAAGAG
CATGTTACAGAGCCACAAGAAAACCCCTTCAACCATCCTGAAATGAGCTAGGATTACTCA
CAGATAAGTTAGACTCTTCAATCCACCAATTTCAGGAGGCAGTGCCTGTTGAAGTTTGTT
CCACCTCAAATTCCTACGAAACCAAACAAGATCAAGACAAAGCAAGTAGCCAAACTGAAT
TCCTCAAGTTGAAGAGTATTACAGTGTGATAAGCCGCTTAAGCCGCCCAATTTCTGGAGG
AATAGGACCTGTTAATTTGTTATTATGCATATCCCTGCCAACCATAATCCATCATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATT
   
Sequence Name 66-K108522-0022-824-F01C-8409
GenBank Accession LN511628 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K108522-0022-824-F01C-8409
TCGGACTGAGCCATTTACATTGAATATGATGGATTATGGTTGGCAGGGATATGCATAATA
ACAAATTAACAGGTCCTATTCCTCCAGAAATTGGGCGGCTTAAGCGGCTTATCACACTGT
AATACTCTTCAACTTGAGGAATTCAGTTTGGCTACTTGCTTTGTCTTGATCTTGTTTGGT
TTCGTAGGAATTTGAGGTGGAACAAACTTCAACAGGCACTGCCTCCTGAAATTGGTGGAT
TGAAGAGTCTAACTTATCTGTGAGTAATCCTAGCTCATTTCAGGATGGTTGAAGGGGTTT
TCTTGTGGCTCTGTAACATGCTCTTAATATTGCAGGTACCTGAGTTTTAACAATTTCAAA
GGAGAAATCCCCAAAGAACTTGCAAATCTCCATGAGCTCCAGTACTTACATATTCAGGAG
AATCATTTTACTGGGCGAATTCCAGCAGAGCTGGGAACATTACAAAAACTTCGCCACTTG
TAATCTTGGACATTC

Last Updated on 06.12.2018 16:11